[3dem] 10nm gold bead source?

[Bruno M. Humbel]

Dear Kelly,

you can easily prepare gold particles at the size you want following the method of Slot and Geuze (I used this protocol for many years)

Slot JW, Geuze HJ. 1981. Sizing of protein A-colloidal gold probes for immunoelectron microscopy. J Cell Biol, 90, 533-536.
http://jcb.rupress.org/content/90/2.toc
http://jcb.rupress.org/content/90/2/533.full.pdf+html

Probably you still can buy it from the Cell Biology group in Utrecht, please contact Dr. Posthuma
g.posthuma at umcutrecht.nl

Or an other good source is Aurion
http://www.aurion.nl/

Good luck,
Bruno

Bruno M. Humbel PhD
Electron Microscopy Facility
University of Lausanne
Biophore
1015 Lausanne
Switzerland

phone: +41 21 6925247
fax: +41 21 6925055
e-mail: Bruno.Humbel at unil.ch



From: Kelly Lee 
Sent: Wednesday, November 19, 2014 1:55 AM
To: 3dem at ncmir.ucsd.edu 
Subject: [3dem] 10nm gold bead source?

Hello!

We've recently been having issues with 10nm gold beads (the type used in tomography) from Sigma forming poorly behaving aggregates. It varies batch to batch; in the past the beads from Sigma have been fine, but not on the last two occasions. 

We typically BSA treat the beads followed by washing. Unfortunately, they have been crashing out in recent preparations (visible in that the solution turns dark purple/grey instead of staying burgundy).


Does anybody have a reliable, recently used source for these types of gold beads? Or any advice on recovering them if they appear to have gone "bad"? 

Many thanks,

Kelly Lee  

________________________________

Kelly Lee, Ph.D.
Department of Medicinal Chemistry
University of Washington

kklee at u.washington.edu
http://faculty.washington.edu/kklee

University of Washington
Department of Medicinal Chemistry
PO Box 357610
Seattle, WA 98195-7610

206-616-3972 (office Health Sciences Bldg H172J)
206-616-3496 (lab Health Sciences Bldg H073)
206-685-3252 (fax)



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