[3dem] 10nm gold bead source?

[dchretie]

Dear Kelly,

We have been using 10 nm gold nanoparticles coated with BSA (Aurion) for a long time and we also occasionally faced aggregation problems. We now use gold nanoparticles coated with a mix-matrix composed of HS-PEG and a peptidol, prepared according to : Duchesne L., Gentili D., Comes-Franchini M. and D.G. Fernig. Robust ligand shells for biological applications of gold nanoparticles . Langmuir, 2008, 24:13572-13580.

We have used these nanoparticles with a variety of samples, and never faced aggregation or aspecific interaction problems. If you (or others) are interested to test them, we can send an aliquot of these nanoparticles. Alternatively, you can prepare your own according to the protocol of Duchesne et al..

Best,

Denis
___________________________
Université de Rennes 1
UMR 6290, équipe TIPs
Campus de Beaulieu, Bât. 13
35042 Rennes Cedex
France

Tel. +33 (0)2 23 23 67 64
http://equipes.igdr.univ-rennes1.fr/fr/tips/ <http://equipes.igdr.univ-rennes1.fr/fr/tips/>
___________________________



> Le 19 nov. 2014 à 01:55, Kelly Lee <kklee at u.washington.edu> a écrit :
> 
> Hello!
> 
> We've recently been having issues with 10nm gold beads (the type used in tomography) from Sigma forming poorly behaving aggregates. It varies batch to batch; in the past the beads from Sigma have been fine, but not on the last two occasions.
> 
> We typically BSA treat the beads followed by washing. Unfortunately, they have been crashing out in recent preparations (visible in that the solution turns dark purple/grey instead of staying burgundy).
> 
> Does anybody have a reliable, recently used source for these types of gold beads? Or any advice on recovering them if they appear to have gone "bad"?
> 
> Many thanks,
> 
> Kelly Lee 
> 
> ________________________________
> 
> Kelly Lee, Ph.D.
> Department of Medicinal Chemistry
> University of Washington
> 
> kklee at u.washington.edu <mailto:kklee at u.washington.edu>
> http://faculty.washington.edu/kklee <http://faculty.washington.edu/kklee>
> 
> University of Washington
> Department of Medicinal Chemistry
> PO Box 357610
> Seattle, WA 98195-7610
> 
> 206-616-3972 (office Health Sciences Bldg H172J)
> 206-616-3496 (lab Health Sciences Bldg H073)
> 206-685-3252 (fax)
> 
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