[3dem] 10nm gold bead source?

[Gibson, Kimberley]

Hi Kelly,

I was wondering if it would be worth it to try putting an aliquot of the aggregating nanobeads into a sonicator bath for a few minutes to see if that would dissociate the aggregating particles.

All the best,

Kimberley Gibson

Post-Graduate Associate, Charles Sindelar Lab

Dept. Molecular Biophysics and Biochemistry
Yale University
SHMC-E15
333 Cedar St.
New Haven, CT
06520-8024
USA

Lab (203) 737-4723
Email: kimberley.gibson at yale.edu
________________________________
From: 3dem-bounces at ncmir.ucsd.edu [3dem-bounces at ncmir.ucsd.edu] on behalf of dchretie [denis.chretien at univ-rennes1.fr]
Sent: November 19, 2014 05:38
To: 3dem at ncmir.ucsd.edu
Subject: Re: [3dem] 10nm gold bead source?

Dear Kelly,

We have been using 10 nm gold nanoparticles coated with BSA (Aurion) for a long time and we also occasionally faced aggregation problems. We now use gold nanoparticles coated with a mix-matrix composed of HS-PEG and a peptidol, prepared according to : Duchesne L., Gentili D., Comes-Franchini M. and D.G. Fernig. Robust ligand shells for biological applications of gold nanoparticles . Langmuir, 2008, 24:13572-13580.

We have used these nanoparticles with a variety of samples, and never faced aggregation or aspecific interaction problems. If you (or others) are interested to test them, we can send an aliquot of these nanoparticles. Alternatively, you can prepare your own according to the protocol of Duchesne et al..

Best,

Denis
___________________________
Université de Rennes 1
UMR 6290, équipe TIPs
Campus de Beaulieu, Bât. 13
35042 Rennes Cedex
France

Tel. +33 (0)2 23 23 67 64
http://equipes.igdr.univ-rennes1.fr/fr/tips/
___________________________



Le 19 nov. 2014 à 01:55, Kelly Lee <kklee at u.washington.edu<mailto:kklee at u.washington.edu>> a écrit :

Hello!

We've recently been having issues with 10nm gold beads (the type used in tomography) from Sigma forming poorly behaving aggregates. It varies batch to batch; in the past the beads from Sigma have been fine, but not on the last two occasions.

We typically BSA treat the beads followed by washing. Unfortunately, they have been crashing out in recent preparations (visible in that the solution turns dark purple/grey instead of staying burgundy).

Does anybody have a reliable, recently used source for these types of gold beads? Or any advice on recovering them if they appear to have gone "bad"?

Many thanks,

Kelly Lee

________________________________

Kelly Lee, Ph.D.
Department of Medicinal Chemistry
University of Washington

kklee at u.washington.edu<mailto:kklee at u.washington.edu>
http://faculty.washington.edu/kklee

University of Washington
Department of Medicinal Chemistry
PO Box 357610
Seattle, WA 98195-7610

206-616-3972 (office Health Sciences Bldg H172J)
206-616-3496 (lab Health Sciences Bldg H073)
206-685-3252 (fax)

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