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<div style="direction: ltr;font-family: Tahoma;color: #000000;font-size: 10pt;">Hi Kelly,
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I was wondering if it would be worth it to try putting an aliquot of the aggregating nanobeads into a sonicator bath for a few minutes to see if that would dissociate the aggregating particles.
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All the best, <br>
<div><br>
<div style="font-family: Tahoma; font-size: 13px;">Kimberley Gibson<br>
<br>
Post-Graduate Associate, Charles Sindelar Lab<br>
<br>
Dept. Molecular Biophysics and Biochemistry<br>
Yale University<br>
SHMC-E15<br>
333 Cedar St.<br>
New Haven, CT<br>
06520-8024<br>
USA<br>
<br>
Lab (203) 737-4723<br>
Email: kimberley.gibson@yale.edu<br>
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<div style="direction: ltr;" id="divRpF780662"><font color="#000000" face="Tahoma" size="2"><b>From:</b> 3dem-bounces@ncmir.ucsd.edu [3dem-bounces@ncmir.ucsd.edu] on behalf of dchretie [denis.chretien@univ-rennes1.fr]<br>
<b>Sent:</b> November 19, 2014 05:38<br>
<b>To:</b> 3dem@ncmir.ucsd.edu<br>
<b>Subject:</b> Re: [3dem] 10nm gold bead source?<br>
</font><br>
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<div>Dear Kelly,
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<div class="">We have been using 10 nm gold nanoparticles coated with BSA (Aurion) for a long time and we also occasionally faced aggregation problems. We now use gold nanoparticles coated with a mix-matrix composed of HS-PEG and a peptidol, prepared according
to : Duchesne L., Gentili D., Comes-Franchini M. and D.G. Fernig. Robust ligand shells for biological applications of gold nanoparticles . Langmuir, 2008, 24:13572-13580.</div>
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<div class="">We have used these nanoparticles with a variety of samples, and never faced aggregation or aspecific interaction problems. If you (or others) are interested to test them, we can send an aliquot of these nanoparticles. Alternatively, you can prepare
your own according to the protocol of Duchesne et al..</div>
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<div class="">Best,</div>
<div class=""><br class="">
</div>
<div class="">Denis</div>
<div class="">
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<div class="">Le 19 nov. 2014 à 01:55, Kelly Lee <<a href="mailto:kklee@u.washington.edu" class="" target="_blank">kklee@u.washington.edu</a>> a écrit :</div>
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<div class="">Hello!</div>
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We've recently been having issues with 10nm gold beads (the type used in tomography) from Sigma forming poorly behaving aggregates. It varies batch to batch; in the past the beads from Sigma have been fine, but not on the last two occasions.
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<div class="">We typically BSA treat the beads followed by washing. Unfortunately, they have been crashing out in recent preparations (visible in that the solution turns dark purple/grey instead of staying burgundy).<br class="">
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<div class="">Does anybody have a reliable, recently used source for these types of gold beads? Or any advice on recovering them if they appear to have gone "bad"?
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<div class="">Many thanks,</div>
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<div class="">Kelly Lee
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<div class="">________________________________</div>
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<div class="">Kelly Lee, Ph.D.</div>
<div class="">Department of Medicinal Chemistry</div>
<div class="">University of Washington</div>
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<div class=""><a href="mailto:kklee@u.washington.edu" class="" target="_blank">kklee@u.washington.edu</a></div>
<div class=""><a href="http://faculty.washington.edu/kklee" class="" target="_blank">http://faculty.washington.edu/kklee</a></div>
<div class=""><br class="">
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<div class="">University of Washington</div>
<div class="">Department of Medicinal Chemistry</div>
<div class="">PO Box 357610</div>
<div class="">Seattle, WA 98195-7610</div>
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</div>
<div class="">206-616-3972 (office Health Sciences Bldg H172J)</div>
<div class="">206-616-3496 (lab Health Sciences Bldg H073)</div>
<div class="">206-685-3252 (fax)</div>
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