[3dem] 10nm gold bead source?

[Kelly Lee]

Hello!

We've recently been having issues with 10nm gold beads (the type used in tomography) from Sigma forming poorly behaving aggregates. It varies batch to batch; in the past the beads from Sigma have been fine, but not on the last two occasions.

We typically BSA treat the beads followed by washing. Unfortunately, they have been crashing out in recent preparations (visible in that the solution turns dark purple/grey instead of staying burgundy).

Does anybody have a reliable, recently used source for these types of gold beads? Or any advice on recovering them if they appear to have gone "bad"?

Many thanks,

Kelly Lee 

________________________________

Kelly Lee, Ph.D.
Department of Medicinal Chemistry
University of Washington

kklee at u.washington.edu
http://faculty.washington.edu/kklee

University of Washington
Department of Medicinal Chemistry
PO Box 357610
Seattle, WA 98195-7610

206-616-3972 (office Health Sciences Bldg H172J)
206-616-3496 (lab Health Sciences Bldg H073)
206-685-3252 (fax)

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