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<DIV>Dear Kelly,</DIV>
<DIV> </DIV>
<DIV>you can easily prepare gold particles at the size you want following the
method of Slot and Geuze (I used this protocol for many years)</DIV>
<DIV> </DIV>
<DIV>Slot JW, Geuze HJ. 1981. Sizing of protein A-colloidal gold probes for
immunoelectron microscopy. J Cell Biol, 90, 533-536.</DIV>
<DIV><A title=http://jcb.rupress.org/content/90/2.toc
href="http://jcb.rupress.org/content/90/2.toc">http://jcb.rupress.org/content/90/2.toc</A></DIV>
<DIV><A title=http://jcb.rupress.org/content/90/2/533.full.pdf+html
href="http://jcb.rupress.org/content/90/2/533.full.pdf+html">http://jcb.rupress.org/content/90/2/533.full.pdf+html</A></DIV>
<DIV> </DIV>
<DIV>Probably you still can buy it from the Cell Biology group in Utrecht,
please contact Dr. Posthuma</DIV>
<DIV><A
href="mailto:g.posthuma@umcutrecht.nl">g.posthuma@umcutrecht.nl</A></DIV>
<DIV> </DIV>
<DIV>Or an other good source is Aurion</DIV>
<DIV><A title=http://www.aurion.nl/
href="http://www.aurion.nl/">http://www.aurion.nl/</A></DIV>
<DIV> </DIV>
<DIV>Good luck,</DIV>
<DIV>Bruno</DIV>
<DIV> </DIV>
<DIV style="FONT-FAMILY: 'Arial'; COLOR: #000000; FONT-SIZE: 10pt">Bruno M.
Humbel PhD<BR>Electron Microscopy Facility<BR>University of
Lausanne<BR>Biophore<BR>1015 Lausanne<BR>Switzerland<BR><BR>phone: +41 21
6925247<BR>fax: +41 21 6925055<BR>e-mail: Bruno.Humbel@unil.ch</DIV>
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<DIV style="font-color: black"><B>From:</B> <A title=kklee@u.washington.edu
href="mailto:kklee@u.washington.edu">Kelly Lee</A> </DIV>
<DIV><B>Sent:</B> Wednesday, November 19, 2014 1:55 AM</DIV>
<DIV><B>To:</B> <A title=3dem@ncmir.ucsd.edu
href="mailto:3dem@ncmir.ucsd.edu">3dem@ncmir.ucsd.edu</A> </DIV>
<DIV><B>Subject:</B> [3dem] 10nm gold bead source?</DIV></DIV></DIV>
<DIV> </DIV></DIV>
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<DIV>Hello!</DIV>
<DIV> </DIV>We've recently been having issues with 10nm gold beads (the
type used in tomography) from Sigma forming poorly behaving aggregates. It
varies batch to batch; in the past the beads from Sigma have been fine, but not
on the last two occasions.
<DIV> </DIV>
<DIV>We typically BSA treat the beads followed by washing. Unfortunately, they
have been crashing out in recent preparations (visible in that the solution
turns dark purple/grey instead of staying burgundy).<BR></DIV>
<DIV> </DIV>
<DIV>Does anybody have a reliable, recently used source for these types of gold
beads? Or any advice on recovering them if they appear to have gone "bad"?
<DIV> </DIV>
<DIV>
<DIV>Many thanks,</DIV>
<DIV> </DIV>
<DIV>Kelly Lee
<DIV>
<DIV> </DIV>
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<DIV>________________________________</DIV>
<DIV> </DIV>
<DIV>Kelly Lee, Ph.D.</DIV>
<DIV>Department of Medicinal Chemistry</DIV>
<DIV>University of Washington</DIV>
<DIV> </DIV>
<DIV><A href="mailto:kklee@u.washington.edu">kklee@u.washington.edu</A></DIV>
<DIV><A
href="http://faculty.washington.edu/kklee">http://faculty.washington.edu/kklee</A></DIV>
<DIV> </DIV>
<DIV>University of Washington</DIV>
<DIV>Department of Medicinal Chemistry</DIV>
<DIV>PO Box 357610</DIV>
<DIV>Seattle, WA 98195-7610</DIV>
<DIV> </DIV>
<DIV>206-616-3972 (office Health Sciences Bldg H172J)</DIV>
<DIV>206-616-3496 (lab Health Sciences Bldg H073)</DIV>
<DIV>206-685-3252 (fax)</DIV></DIV></SPAN></DIV></SPAN></SPAN></DIV>
<DIV> </DIV></DIV></DIV></DIV></DIV>
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