[3dem] Error with RELION, autopick for filaments

[Irene Del Mar Farinas Lucas]

Hello!

I'm kind of new to Relion, so I've been learning bit by bit, but now I'm stuck. I've picked particles using the Helix feature until I got pretty good classes. I used those to train a model in Topaz and then used that model to do autopicking. However, when I try to extract those particles, the job terminates with errors. First, I was getting the error: “ERROR: helix.cpp::convertHelicalTubeCoordsToMetaDataTable(): Input coordinates should be in pairs!”

I switched off the option for "Coordinates are start-end only?" and now I'm getting the error: “ERROR: helix.cpp::convertHelicalSegmentCoordsToMetaDataTable(): Prior information of helical segments is missing in micrographs.” The problem is probably due to the settings in Topaz and not the extraction, but I'm not sure what the exact issue is.
Any help is welcomed!
Thanks,
Irene Farinas
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