[3dem] Inconsistent Blotting with GP2

[Sen, Anindito]

HI Liz,

Is the chamber humidity too high (the filter papers get wet?).

Stay safe & Regards

Anindito Sen. Ph.D

Microscopy and Imaging Center
Texas A&M University
301 Old Main Dr
College Station, TX 77843-2257

Office: ILSB Room 1133
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From: 3dem <3dem-bounces at ncmir.ucsd.edu> on behalf of Liz Montabana <liz.montabana at czii.org>
Date: Wednesday, August 7, 2024 at 6:59 PM
To: 3dem at ncmir.ucsd.edu <3dem at ncmir.ucsd.edu>
Subject: [3dem] Inconsistent Blotting with GP2
Hi All We are seeing a lot of inconsistent blotting with our GP2 plunge freezer (The longer we blot, the thicker our grids are, among other odd behavior) - and we wanted to ask others' experience to see if we could pinpoint any cause or
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Hi All

We are seeing a lot of inconsistent blotting with our GP2 plunge freezer (The longer we blot, the thicker our grids are, among other odd behavior) - and we wanted to ask others' experience to see if we could pinpoint any cause or feedback we could provide Leica to enhance the performance.

Please share your trade tips and secrets for wrangling the GP2 - We've had experience with several other GP2s and none of them shared this behavior. Our main samples are whole cells grown on grids.

Thanks
Liz Montabana
Chan Zuckerberg Imaging Institute
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