[3dem] Inconsistent Blotting with GP2

[Ivica Odorčić]

What we found works very well is to cut out a piece of thin plastic (such as the clear cover of a plastic paper folder) with a slightly smaller diameter than the filter paper (also cut the hole). Then glue the paper to the plastic with a glue stick (just enough glue to hold them together). Mount it in the plunger with the paper facing the grid and this will keep the paper flat at constant 100% humidity. After plunging, you can easily separate the paper and plastic, wash the plastic and reuse it.

Ivica Odorcic


> ----------------------------------------------------------------------
> 
> Message: 1
> Date: Fri, 9 Aug 2024 16:02:17 -0700
> From: Daniel Asarnow <asarnow at msg.ucsf.edu>
> To: David Bulkley <davidbulkley at gmail.com>
> Cc: 3dem at ncmir.ucsd.edu
> Subject: Re: [3dem] 3dem Digest, Vol 204, Issue 11
> Message-ID:
> 	<CALiNCbYT0JNzEC+FxxrwXqVRnOpwFsLsWHKQNgs1G3t62xc5mQ at mail.gmail.com>
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> Or one could 3D print a wider backing plate in nylon or ABS.
> 
> -da