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<p class="MsoNormal"><span style="font-size:14.0pt;font-family:"Times New Roman",serif">HI Liz,<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:14.0pt;font-family:"Times New Roman",serif"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:14.0pt;font-family:"Times New Roman",serif">Is the chamber humidity too high (the filter papers get wet?).
<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:12.0pt;font-family:"Times New Roman",serif;color:black">Stay safe & Regards<o:p></o:p></span></p>
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<p class="MsoNormal"><b><span style="font-size:10.0pt;font-family:"Tahoma",sans-serif;color:#006FC9">Anindito Sen. Ph.D</span></b><span style="font-size:12.0pt;font-family:"Times New Roman",serif;color:black"><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Tahoma",sans-serif;color:#006FC9">Microscopy and Imaging Center </span><span style="font-size:10.0pt;font-family:"Tahoma",sans-serif;color:black"><br>
</span><span style="font-size:10.0pt;font-family:"Tahoma",sans-serif;color:#006FC9">Texas A&M University </span><span style="font-size:12.0pt;font-family:"Times New Roman",serif;color:black"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Tahoma",sans-serif;color:#006FC9">301 Old Main Dr</span><span style="font-size:12.0pt;font-family:"Times New Roman",serif;color:black"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Tahoma",sans-serif;color:#006FC9">College Station, TX 77843-2257</span><span style="font-size:12.0pt;font-family:"Times New Roman",serif;color:black"><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Tahoma",sans-serif;color:#006FC9">Office: ILSB Room 1133</span><span style="font-size:12.0pt;font-family:"Times New Roman",serif;color:black"><a href="https://urldefense.com/v3/__http://microscopy.tamu.edu/__;!!Mih3wA!EScYo9GmAITBjF4H0ZqnB6q-l3b7s72KklWzC24V7YgNVActnqSSTEhHU7FaRZibo9Z2l9RYYjUdkMRGAmU$"><span style="font-size:10.0pt;font-family:"Tahoma",sans-serif;color:#006FC9"><br>
</span></a></span><span style="font-size:10.0pt;font-family:"Tahoma",sans-serif;color:#006FC9">Tel. 979-458-9881</span><span style="font-size:10.0pt;font-family:"Tahoma",sans-serif;color:black"><br>
</span><span style="font-size:10.0pt;font-family:"Tahoma",sans-serif;color:#006FC9">fax: 979-847-8933</span><span style="font-size:12.0pt;font-family:"Times New Roman",serif;color:black"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:12.0pt;font-family:"Times New Roman",serif;color:black"><a href="https://urldefense.com/v3/__http://microscopy.tamu.edu/__;!!Mih3wA!EScYo9GmAITBjF4H0ZqnB6q-l3b7s72KklWzC24V7YgNVActnqSSTEhHU7FaRZibo9Z2l9RYYjUdkMRGAmU$"><span style="font-family:"Calibri",sans-serif;color:#006FC9">http://microscopy.tamu.edu</span></a><o:p></o:p></span></p>
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<p class="MsoNormal" style="margin-bottom:12.0pt"><b><span style="font-size:12.0pt;color:black">From:
</span></b><span style="font-size:12.0pt;color:black">3dem <3dem-bounces@ncmir.ucsd.edu> on behalf of Liz Montabana <liz.montabana@czii.org><br>
<b>Date: </b>Wednesday, August 7, 2024 at 6:59 PM<br>
<b>To: </b>3dem@ncmir.ucsd.edu <3dem@ncmir.ucsd.edu><br>
<b>Subject: </b>[3dem] Inconsistent Blotting with GP2<o:p></o:p></span></p>
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<p class="MsoNormal" style="mso-line-height-alt:.75pt"><span style="font-size:1.0pt;color:white">Hi All We are seeing a lot of inconsistent blotting with our GP2 plunge freezer (The longer we blot, the thicker our grids are, among other odd behavior) - and
we wanted to ask others' experience to see if we could pinpoint any cause or<o:p></o:p></span></p>
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<p class="MsoNormal">Hi All<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">We are seeing a lot of inconsistent blotting with our GP2 plunge freezer (The longer we blot, the thicker our grids are, among other odd behavior) - and we wanted to ask others' experience to see if we could pinpoint any cause or feedback
we could provide Leica to enhance the performance.<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">Please share your trade tips and secrets for wrangling the GP2 - We've had experience with several other GP2s and none of them shared this behavior. Our main samples are whole cells grown on grids.<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">Thanks<o:p></o:p></p>
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<p class="MsoNormal">Liz Montabana<o:p></o:p></p>
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<p class="MsoNormal">Chan Zuckerberg Imaging Institute<o:p></o:p></p>
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