[3dem] Inconsistent Blotting with GP2

[Michael Elbaum]

Hi Liz,     something that comes to mind is a fault that maybe the blotting paper doesn't turn. You end up blotting with the same wet area. (yes, this happened to me and I didn't pay attention till too late) You can mark the paper and just run it through the motions without a tweezer, to make sure it turns on every cycle. Something to rule out, at least...

regards,

Michael

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From: 3dem <3dem-bounces at ncmir.ucsd.edu> on behalf of Liz Montabana <liz.montabana at czii.org>
Sent: Thursday, August 8, 2024 2:59:01 AM
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Subject: [3dem] Inconsistent Blotting with GP2

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Hi All

We are seeing a lot of inconsistent blotting with our GP2 plunge freezer (The longer we blot, the thicker our grids are, among other odd behavior) - and we wanted to ask others' experience to see if we could pinpoint any cause or feedback we could provide Leica to enhance the performance.

Please share your trade tips and secrets for wrangling the GP2 - We've had experience with several other GP2s and none of them shared this behavior. Our main samples are whole cells grown on grids.

Thanks
Liz Montabana
Chan Zuckerberg Imaging Institute
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