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<p>Hi Liz, something that comes to mind is a fault that maybe the blotting paper doesn't turn. You end up blotting with the same wet area. (yes, this happened to me and I didn't pay attention till too late) You can mark the paper and just run it through
the motions without a tweezer, to make sure it turns on every cycle. Something to rule out, at least...</p>
<p>regards,</p>
<p>Michael<br>
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<div id="divRplyFwdMsg" dir="ltr"><font face="Calibri, sans-serif" style="font-size:11pt" color="#000000"><b>From:</b> 3dem <3dem-bounces@ncmir.ucsd.edu> on behalf of Liz Montabana <liz.montabana@czii.org><br>
<b>Sent:</b> Thursday, August 8, 2024 2:59:01 AM<br>
<b>To:</b> 3dem@ncmir.ucsd.edu<br>
<b>Subject:</b> [3dem] Inconsistent Blotting with GP2</font>
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<div dir="ltr">Hi All
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<div>We are seeing a lot of inconsistent blotting with our GP2 plunge freezer (The longer we blot, the thicker our grids are, among other odd behavior) - and we wanted to ask others' experience to see if we could pinpoint any cause or feedback we could provide
Leica to enhance the performance.</div>
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<div>Please share your trade tips and secrets for wrangling the GP2 - We've had experience with several other GP2s and none of them shared this behavior. Our main samples are whole cells grown on grids.</div>
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<div>Thanks</div>
<div>Liz Montabana</div>
<div>Chan Zuckerberg Imaging Institute</div>
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