[3dem] Inconsistent Blotting with GP2

Liz Montabana liz.montabana at czii.org
Wed Aug 7 16:59:01 PDT 2024


Hi All

We are seeing a lot of inconsistent blotting with our GP2 plunge freezer
(The longer we blot, the thicker our grids are, among other odd behavior) -
and we wanted to ask others' experience to see if we could pinpoint any
cause or feedback we could provide Leica to enhance the performance.

Please share your trade tips and secrets for wrangling the GP2 - We've had
experience with several other GP2s and none of them shared this behavior.
Our main samples are whole cells grown on grids.

Thanks
Liz Montabana
Chan Zuckerberg Imaging Institute
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