[3dem] tilted transfer functions?
Sjors Scheres
scheres at mrc-lmb.cam.ac.uk
Tue Mar 1 02:38:13 PST 2022
Hi David,
If you do your subtomogram averaging in relion-4, estimating these
high-tilt CTFs over the entire micrographs is probably not that
important, provided that you optimise defoci in a CTF refinement job
after averaging. There, the entire tilt and sample geometry is taken
into account.
HTH,
Sjors
On 28/02/2022 22:41, Morgan, David Gene wrote:
> Hi,
>
> What's the best way to evaluate the CTF of an image that has a
> significant amount of tilt?
>
> --
> politics is more difficult than physics.
> A. Einstein
>
> David Gene Morgan
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