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<p>Hi David,</p>
<p>If you do your subtomogram averaging in relion-4, estimating
these high-tilt CTFs over the entire micrographs is probably not
that important, provided that you optimise defoci in a CTF
refinement job after averaging. There, the entire tilt and sample
geometry is taken into account.</p>
<p>HTH,</p>
<p>Sjors</p>
<p><br>
</p>
<div class="moz-cite-prefix">On 28/02/2022 22:41, Morgan, David Gene
wrote:<br>
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Hi,</div>
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What's the best way to evaluate the CTF of an image that has a
significant amount of tilt?<br>
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David Gene Morgan<br>
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