[3dem] Leica EM GP2 Plunge Freezer - Problems shooting with humidifier chamber

Thu Jan 14 12:08:53 PST 2021

Dear Vanessa,
If you have partial humidification but not full humidification, you may have a failure of one of the two fans. Remove the baffle cover and then raise the chamber and fill the water tank. Recording a cellphone video is a good way to see if the fans are spinning or not, as it may be hard with the naked eye.
Best,
JTK

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Today's Topics:

   1. Leica EM GP2 Plunge Freezer - Problems shooting with
      humidifier chamber (Vanessa Carvalho)
   2. cryo-EM postdoc at KTH Royal Institute of Technology
      (Joakim And?n)

----------------------------------------------------------------------

Message: 1
Date: Thu, 14 Jan 2021 15:53:11 +0100
From: Vanessa Carvalho <vanessaisabeldiascarvalho at gmail.com>
To: 3dem at ncmir.ucsd.edu
Subject: [3dem] Leica EM GP2 Plunge Freezer - Problems shooting with
        humidifier chamber
Message-ID:
        <CAH9iyPUiFHn0JVh2NXSQgcOJRwQw_7uM3MwDbN_rb5cJJYPeSA at mail.gmail.com>
Content-Type: text/plain; charset="utf-8"

Dear 3DEM community,

I would like to ask you about your experience using the Leica EM GP2 Plunge
Freezer.

At our facility is the third time that we need to replace the humidifier
chamber, what happens is that after a couple of months the machine stops
being able to reach the 99% of humidity in the chamber and the mist is
completely gone or going very slowly. It is only able to go around 80% and
sometimes after leaving it working for a lot of the time it is possible to
reach 94% but then the humidifier chamber needs to be replaced, which is
quite expensive.

We have informed Leica about this, and we were advised to decrease the
amount of water introduced to 70 mL and do it slowly. At our facility we
are all experienced users, so we are also sure that this is not a "first
user misusing the machine" and that the instructions are being followed.

Has someone else experienced this problem? Could you fix it with any
approach that can help to prevent this problem?

Thank you in advance for sharing your experience.

Wishes of a great New Year to all and stay safe!

Best,
*Vanessa Carvalho*, PhD
*Team manager, Strategic Research & Marketing, EM services*
*Vironova AB*
G?vlegatan 22, 113 30 Stockholm, Sweden
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vanessa.carvalho at vironova.com <firstname.lastname at vironova.com>
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------------------------------

Message: 2
Date: Thu, 14 Jan 2021 14:29:08 +0100
From: Joakim And?n <janden at kth.se>
To: 3dem at ncmir.ucsd.edu
Cc: P?r Kurlberg <kurlberg at kth.se>, Ozan ?ktem <ozan at kth.se>
Subject: [3dem] cryo-EM postdoc at KTH Royal Institute of Technology
Message-ID: <ee3e64e9-0d48-5eac-1721-2c19509731c3 at kth.se>
Content-Type: text/plain; charset="utf-8"; Format="flowed"

Dear all,

The Department of Mathematics at KTH Royal Institute of Technology is
accepting applications for a postdoc position in computational
single-particle cryo-EM funded by Brummer & Partners MathDataLab. The
project will focus on proving formal uniqueness for Ewald
sphere-corrected reconstruction and methods for direct reconstruction of
the atomic model from projection images using deep neural networks. More
information is found here:

    https://urldefense.com/v3/__https://nam02.safelinks.protection.outlook.com/?url=https*3A*2F*2Furldefense.com*2Fv3*2F__https*3A*2F*2Fwww.kth.se*2Fpolopoly_fs*2F1.1038325.1608296349!*2Fmdl_anden_kurlberg_oktem.pdf__*3B!!Mih3wA!QS6sqognOCbGGK98sNvWyutqhgMZVb_gbcs8v0djGBKgKvQ0mAJ0LX0m27_M3p8SIw*24&data=04*7C01*7Cjason.kaelber*40rutgers.edu*7Cf78d2900402f4e465b0b08d8b8c701f0*7Cb92d2b234d35447093ff69aca6632ffe*7C1*7C0*7C637462512159601865*7CUnknown*7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0*3D*7C1000&sdata=gyI*2F88xT3eet0FHGJoeCBsnReufsGleCtJsP5jKEnpU*3D&reserved=0__;JSUlJSUlJSUlJSUlJSUlJSUlJSUlJSUlJSUl!!Mih3wA!VQ2rFxhUgru2CXxptoJY1yC_McR1I0UZ1IhE1p5b6f_JH9bLdv0MLcoWjGTDt2AN1g$ 

Candidates with previous experience in single-particle cryo-EM, inverse
problems, complex analysis, representation theory, or machine learning
are invited to apply. Significant programming experience will be
considered as an additional merit.

To apply, please see:

    https://urldefense.com/v3/__https://nam02.safelinks.protection.outlook.com/?url=https*3A*2F*2Furldefense.com*2Fv3*2F__https*3A*2F*2Fwww.kth.se*2Fen*2Fom*2Fwork-at-kth*2Flediga-jobb*2Fwhat*3Ajob*2FjobID*3A369575*2Fwhere*3A4*2F__*3B!!Mih3wA!QS6sqognOCbGGK98sNvWyutqhgMZVb_gbcs8v0djGBKgKvQ0mAJ0LX0m27_g00Q-7w*24&data=04*7C01*7Cjason.kaelber*40rutgers.edu*7Cf78d2900402f4e465b0b08d8b8c701f0*7Cb92d2b234d35447093ff69aca6632ffe*7C1*7C0*7C637462512159611856*7CUnknown*7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0*3D*7C1000&sdata=Vdh7elng*2FO*2F2neZRNrzyDWTMik41UyeS*2Fq7tJ*2B3YyAc*3D&reserved=0__;JSUlJSUlJSUlJSUlJSUlJSUlJSUlJSUlJSUlJSUlJSUlJSUlJSU!!Mih3wA!VQ2rFxhUgru2CXxptoJY1yC_McR1I0UZ1IhE1p5b6f_JH9bLdv0MLcoWjGSTcFwHCQ$ 

Make sure to specify that you are applying for the project ?Uniqueness
and atomic modelling in cryo-electron microscopy?. The deadline is
January 20th.

Best,

Joakim And?n, Ozan ?ktem, and P?r Kurlberg
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