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Dear Vanessa,</div>
<div style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 12pt; color: rgb(0, 0, 0);">
If you have partial humidification but not full humidification, you may have a failure of one of the two fans. Remove the baffle cover and then raise the chamber and fill the water tank. Recording a cellphone video is a good way to see if the fans are spinning
or not, as it may be hard with the naked eye.</div>
<div style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 12pt; color: rgb(0, 0, 0);">
Best,</div>
<div style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 12pt; color: rgb(0, 0, 0);">
JTK<br>
</div>
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<div id="divRplyFwdMsg" dir="ltr"><font style="font-size:11pt" face="Calibri, sans-serif" color="#000000"><b>From:</b> 3dem <3dem-bounces@ncmir.ucsd.edu> on behalf of 3dem-request@ncmir.ucsd.edu <3dem-request@ncmir.ucsd.edu><br>
<b>Sent:</b> Thursday, January 14, 2021 3:00 PM<br>
<b>To:</b> 3dem@ncmir.ucsd.edu <3dem@ncmir.ucsd.edu><br>
<b>Subject:</b> 3dem Digest, Vol 161, Issue 16</font>
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Today's Topics:<br>
<br>
1. Leica EM GP2 Plunge Freezer - Problems shooting with<br>
humidifier chamber (Vanessa Carvalho)<br>
2. cryo-EM postdoc at KTH Royal Institute of Technology<br>
(Joakim And?n)<br>
<br>
<br>
----------------------------------------------------------------------<br>
<br>
Message: 1<br>
Date: Thu, 14 Jan 2021 15:53:11 +0100<br>
From: Vanessa Carvalho <vanessaisabeldiascarvalho@gmail.com><br>
To: 3dem@ncmir.ucsd.edu<br>
Subject: [3dem] Leica EM GP2 Plunge Freezer - Problems shooting with<br>
humidifier chamber<br>
Message-ID:<br>
<CAH9iyPUiFHn0JVh2NXSQgcOJRwQw_7uM3MwDbN_rb5cJJYPeSA@mail.gmail.com><br>
Content-Type: text/plain; charset="utf-8"<br>
<br>
Dear 3DEM community,<br>
<br>
<br>
<br>
I would like to ask you about your experience using the Leica EM GP2 Plunge<br>
Freezer.<br>
<br>
At our facility is the third time that we need to replace the humidifier<br>
chamber, what happens is that after a couple of months the machine stops<br>
being able to reach the 99% of humidity in the chamber and the mist is<br>
completely gone or going very slowly. It is only able to go around 80% and<br>
sometimes after leaving it working for a lot of the time it is possible to<br>
reach 94% but then the humidifier chamber needs to be replaced, which is<br>
quite expensive.<br>
<br>
We have informed Leica about this, and we were advised to decrease the<br>
amount of water introduced to 70 mL and do it slowly. At our facility we<br>
are all experienced users, so we are also sure that this is not a "first<br>
user misusing the machine" and that the instructions are being followed.<br>
<br>
<br>
<br>
Has someone else experienced this problem? Could you fix it with any<br>
approach that can help to prevent this problem?<br>
<br>
<br>
<br>
Thank you in advance for sharing your experience.<br>
<br>
<br>
<br>
Wishes of a great New Year to all and stay safe!<br>
<br>
Best,<br>
*Vanessa Carvalho*, PhD<br>
*Team manager, Strategic Research & Marketing, EM services*<br>
*Vironova AB*<br>
G?vlegatan 22, 113 30 Stockholm, Sweden<br>
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Mobile +46 (0) 70 859 15 88 <+46%2011%20222%2033%2044><br>
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<br>
------------------------------<br>
<br>
Message: 2<br>
Date: Thu, 14 Jan 2021 14:29:08 +0100<br>
From: Joakim And?n <janden@kth.se><br>
To: 3dem@ncmir.ucsd.edu<br>
Cc: P?r Kurlberg <kurlberg@kth.se>, Ozan ?ktem <ozan@kth.se><br>
Subject: [3dem] cryo-EM postdoc at KTH Royal Institute of Technology<br>
Message-ID: <ee3e64e9-0d48-5eac-1721-2c19509731c3@kth.se><br>
Content-Type: text/plain; charset="utf-8"; Format="flowed"<br>
<br>
Dear all,<br>
<br>
The Department of Mathematics at KTH Royal Institute of Technology is <br>
accepting applications for a postdoc position in computational <br>
single-particle cryo-EM funded by Brummer & Partners MathDataLab. The <br>
project will focus on proving formal uniqueness for Ewald <br>
sphere-corrected reconstruction and methods for direct reconstruction of <br>
the atomic model from projection images using deep neural networks. More <br>
information is found here:<br>
<br>
<a href="https://urldefense.com/v3/__https://nam02.safelinks.protection.outlook.com/?url=https*3A*2F*2Furldefense.com*2Fv3*2F__https*3A*2F*2Fwww.kth.se*2Fpolopoly_fs*2F1.1038325.1608296349!*2Fmdl_anden_kurlberg_oktem.pdf__*3B!!Mih3wA!QS6sqognOCbGGK98sNvWyutqhgMZVb_gbcs8v0djGBKgKvQ0mAJ0LX0m27_M3p8SIw*24&data=04*7C01*7Cjason.kaelber*40rutgers.edu*7Cf78d2900402f4e465b0b08d8b8c701f0*7Cb92d2b234d35447093ff69aca6632ffe*7C1*7C0*7C637462512159601865*7CUnknown*7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0*3D*7C1000&sdata=gyI*2F88xT3eet0FHGJoeCBsnReufsGleCtJsP5jKEnpU*3D&reserved=0__;JSUlJSUlJSUlJSUlJSUlJSUlJSUlJSUlJSUl!!Mih3wA!VQ2rFxhUgru2CXxptoJY1yC_McR1I0UZ1IhE1p5b6f_JH9bLdv0MLcoWjGTDt2AN1g$">
https://nam02.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.com%2Fv3%2F__https%3A%2F%2Fwww.kth.se%2Fpolopoly_fs%2F1.1038325.1608296349!%2Fmdl_anden_kurlberg_oktem.pdf__%3B!!Mih3wA!QS6sqognOCbGGK98sNvWyutqhgMZVb_gbcs8v0djGBKgKvQ0mAJ0LX0m27_M3p8SIw%24&data=04%7C01%7Cjason.kaelber%40rutgers.edu%7Cf78d2900402f4e465b0b08d8b8c701f0%7Cb92d2b234d35447093ff69aca6632ffe%7C1%7C0%7C637462512159601865%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=gyI%2F88xT3eet0FHGJoeCBsnReufsGleCtJsP5jKEnpU%3D&reserved=0</a>
<br>
<br>
Candidates with previous experience in single-particle cryo-EM, inverse <br>
problems, complex analysis, representation theory, or machine learning <br>
are invited to apply. Significant programming experience will be <br>
considered as an additional merit.<br>
<br>
To apply, please see:<br>
<br>
<a href="https://urldefense.com/v3/__https://nam02.safelinks.protection.outlook.com/?url=https*3A*2F*2Furldefense.com*2Fv3*2F__https*3A*2F*2Fwww.kth.se*2Fen*2Fom*2Fwork-at-kth*2Flediga-jobb*2Fwhat*3Ajob*2FjobID*3A369575*2Fwhere*3A4*2F__*3B!!Mih3wA!QS6sqognOCbGGK98sNvWyutqhgMZVb_gbcs8v0djGBKgKvQ0mAJ0LX0m27_g00Q-7w*24&data=04*7C01*7Cjason.kaelber*40rutgers.edu*7Cf78d2900402f4e465b0b08d8b8c701f0*7Cb92d2b234d35447093ff69aca6632ffe*7C1*7C0*7C637462512159611856*7CUnknown*7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0*3D*7C1000&sdata=Vdh7elng*2FO*2F2neZRNrzyDWTMik41UyeS*2Fq7tJ*2B3YyAc*3D&reserved=0__;JSUlJSUlJSUlJSUlJSUlJSUlJSUlJSUlJSUlJSUlJSUlJSUlJSU!!Mih3wA!VQ2rFxhUgru2CXxptoJY1yC_McR1I0UZ1IhE1p5b6f_JH9bLdv0MLcoWjGSTcFwHCQ$">
https://nam02.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.com%2Fv3%2F__https%3A%2F%2Fwww.kth.se%2Fen%2Fom%2Fwork-at-kth%2Flediga-jobb%2Fwhat%3Ajob%2FjobID%3A369575%2Fwhere%3A4%2F__%3B!!Mih3wA!QS6sqognOCbGGK98sNvWyutqhgMZVb_gbcs8v0djGBKgKvQ0mAJ0LX0m27_g00Q-7w%24&data=04%7C01%7Cjason.kaelber%40rutgers.edu%7Cf78d2900402f4e465b0b08d8b8c701f0%7Cb92d2b234d35447093ff69aca6632ffe%7C1%7C0%7C637462512159611856%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=Vdh7elng%2FO%2F2neZRNrzyDWTMik41UyeS%2Fq7tJ%2B3YyAc%3D&reserved=0</a>
<br>
<br>
Make sure to specify that you are applying for the project ?Uniqueness <br>
and atomic modelling in cryo-electron microscopy?. The deadline is <br>
January 20th.<br>
<br>
Best,<br>
<br>
Joakim And?n, Ozan ?ktem, and P?r Kurlberg<br>
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End of 3dem Digest, Vol 161, Issue 16<br>
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