[3dem] K3 optimal dose rate & F20 parallel illumination

Thu Feb 14 10:44:38 PST 2019

Hi Garry,

Yes, you'll want to work in nP. We're encountering the same issue with our new Arctica and had a Thermo apps person here helping us with validation. We're using this awesome paper as our work guide:

https://www.biorxiv.org/content/10.1101/141994v1

I'd also check notes from UMass and LBNL. Lots of helpful hints from various groups.

HTH,

Mariena

Sent from my Game Boy

On Feb 14, 2019, at 1:36 PM, Garry P Morgan <Garry.Morgan at colorado.edu<mailto:Garry.Morgan at colorado.edu>> wrote:

Hello all,

i’m looking for some info on the optimal dose rate for acquiring K3 movie stacks.  i would appreciate it if any of you could send me your optimum acquisition parameters for imaging cry-samples for SPA…ie, dose rate, best frame rate/number of frames, etc.

i’m also trying to figure out how to setup my low dose so that we have parallel illumination for our record images (on an FEI Tecnai F20). the problem i see is that in microprobe the beam is spread much too wide to image/expose a single hole at a time at our record mag. is it advisable to work in nano probe to get a smaller beam that is parallel? any advice on this would be appreciated.

thanks,
Garry

________________________________
Garry Morgan
Director  —  Boulder EM Services —  Department of MCD Biology
Campus Box 347  —  University of Colorado  —  Boulder, CO 80309
phone:  303-492-8402

_______________________________________________
3dem mailing list
3dem at ncmir.ucsd.edu<mailto:3dem at ncmir.ucsd.edu>
https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem
-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://mail.ncmir.ucsd.edu/pipermail/3dem/attachments/20190214/fef8f775/attachment.html>