[3dem] K3 optimal dose rate & F20 parallel illumination

[Sen, Anindito]

HI,

We have a similar issue. Thanks for the information and the paper.

Regards

Anindito Sen. Ph.D

Microscopy and Imaging Center
Texas A&M University
301 Old Main Dr
College Station, TX 77843-2257

Office: ILSB Room 1133
<http://microscopy.tamu.edu/>Tel. 979-458-9881
fax: 979-847-8933
http://microscopy.tamu.edu<http://microscopy.tamu.edu/>




From: 3dem <3dem-bounces at ncmir.ucsd.edu> on behalf of Mariena Silvestry Ramos <ms3289 at cornell.edu>
Date: Thursday, February 14, 2019 at 12:44 PM
To: Garry P Morgan <Garry.Morgan at colorado.edu>, "3dem at ncmir.ucsd.edu" <3dem at ncmir.ucsd.edu>
Subject: Re: [3dem] K3 optimal dose rate & F20 parallel illumination

Hi Garry,

Yes, you'll want to work in nP. We're encountering the same issue with our new Arctica and had a Thermo apps person here helping us with validation. We're using this awesome paper as our work guide:

https://www.biorxiv.org/content/10.1101/141994v1<https://urldefense.proofpoint.com/v2/url?u=https-3A__www.biorxiv.org_content_10.1101_141994v1&d=DwMGaQ&c=ODFT-G5SujMiGrKuoJJjVg&r=d0bn34cruVB1VWavzhgmaA&m=7V_jh_i0cwQqkNDnEy0qG2aMagwoNQJNmxk-dnBezKY&s=4d2J5owJIAZm8leQXSofAsGmODnGqaLS4zYVXXecBX8&e=>

I'd also check notes from UMass and LBNL. Lots of helpful hints from various groups.

HTH,

Mariena

Sent from my Game Boy

On Feb 14, 2019, at 1:36 PM, Garry P Morgan <Garry.Morgan at colorado.edu<mailto:Garry.Morgan at colorado.edu>> wrote:
Hello all,

i’m looking for some info on the optimal dose rate for acquiring K3 movie stacks.  i would appreciate it if any of you could send me your optimum acquisition parameters for imaging cry-samples for SPA…ie, dose rate, best frame rate/number of frames, etc.

i’m also trying to figure out how to setup my low dose so that we have parallel illumination for our record images (on an FEI Tecnai F20). the problem i see is that in microprobe the beam is spread much too wide to image/expose a single hole at a time at our record mag. is it advisable to work in nano probe to get a smaller beam that is parallel? any advice on this would be appreciated.

thanks,
Garry



________________________________
Garry Morgan
Director  —  Boulder EM Services —  Department of MCD Biology
Campus Box 347  —  University of Colorado  —  Boulder, CO 80309
phone:  303-492-8402

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