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Hi Garry,
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<div>Yes, you'll want to work in nP. We're encountering the same issue with our new Arctica and had a Thermo apps person here helping us with validation. We're using this awesome paper as our work guide:</div>
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<div><a href="https://www.biorxiv.org/content/10.1101/141994v1">https://www.biorxiv.org/content/10.1101/141994v1</a><br>
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I'd also check notes from UMass and LBNL. Lots of helpful hints from various groups. </div>
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<div>HTH,</div>
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<div>Mariena<br>
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<div id="AppleMailSignature" dir="ltr">Sent from my Game Boy</div>
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On Feb 14, 2019, at 1:36 PM, Garry P Morgan <<a href="mailto:Garry.Morgan@colorado.edu">Garry.Morgan@colorado.edu</a>> wrote:<br>
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<div dir="ltr"><span>Hello all,</span><br>
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<span>i’m looking for some info on the optimal dose rate for acquiring K3 movie stacks. i would appreciate it if any of you could send me your optimum acquisition parameters for imaging cry-samples for SPA…ie, dose rate, best frame rate/number of frames, etc.</span><br>
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<span>i’m also trying to figure out how to setup my low dose so that we have parallel illumination for our record images (on an FEI Tecnai F20). the problem i see is that in microprobe the beam is spread much too wide to image/expose a single hole at a time
at our record mag. is it advisable to work in nano probe to get a smaller beam that is parallel? any advice on this would be appreciated.</span><br>
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<span>thanks,</span><br>
<span>Garry</span><br>
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<span>________________________________</span><br>
<span>Garry Morgan</span><br>
<span>Director — Boulder EM Services — Department of MCD Biology</span><br>
<span>Campus Box 347 — University of Colorado — Boulder, CO 80309</span><br>
<span>phone: 303-492-8402</span><br>
<span></span><br>
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