[3dem] Plasmon imaging - EPU 3.9.1 with K3/BioQuantum

[Andrea Nans]

Hi Piotr,

In image mode the cross-over correction is the same as diffraction shift
and vice versa, as it uses the same image deflection coils as the
image/diffraction shift. You could bind the diffraction shift to
multifunction knobs and adjust them in LM EFTEM. I've just tested this on
my system with a K2 and BioQuantum and it seems to work. You can monitor
the "user diff shift" parameter in system status to keep track of the
diffraction shift.

Best,

Andrea
Cryo-EM Facility Manager
Francis Crick Institute

On Fri, Nov 22, 2024 at 6:35 AM Piotr Szwedziak <piotr.szwedziak at zmb.uzh.ch>
wrote:

> Dear cryoEM colleagues,
>
> we have recently upgraded to the latest EPU version (3.9.1). Our system
> (Titan Krios G3i) is equipped with a K3 camera and a BioQuantum energy
> filter and we wanted to take advantage of the plasmon imaging that is now
> embedded in EPU. However, when we center ZLP at data acquisition
> magnification (SA, 130kx), the slit is miscentered in the LM range that is
> routinely used for Atlas/Grid Square magnifications. For some reason, the
> cross-over correction is not available in direct alignments once in the LM
> range therefore I can not adjust it. My question is if anyone has
> experienced something similar and has ideas on how to resolve this?  We
> have opened a ticket with TFS but I thought I would ask the community in
> the meantime.
>
> best wishes and many thanks,
>
> Piotr
>
>
>
>
>
>
>
> --
> Piotr Szwedziak, PhD
> Center for Microscopy and Image Analysis
> University of Zurich
> Winterthurerstrasse 190
> CH-8057 Zurich
> Switzerland
>
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