[3dem] Topaz auto pick relion-5 FOM for filament picking issue

[Sjors Scheres]

Hi Virajith,

This works slightly different from single particle topaz: you need to 
specify the FOM threshold on the GUI to be used for the extraction of 
entire filaments; you can no longer play with the threshold afterwards 
in extraction, as you can for single-particles. You can use the extra 
command -fp to get some visual feedback for micrographs, which can help 
to decide the threshold.

HTH,

Sjors


On 07/11/2024 12:22, virajith cryo-em wrote:
> CAUTION: This email originated from outside of the LMB:
> *.-3dem-bounces at ncmir.ucsd.edu-.*
> Do not click links or open attachments unless you recognize the sender 
> and know the content is safe.
> If you think this is a phishing email, please forward it to 
> phishing at mrc-lmb.cam.ac.uk
>
>
> --
>
> Hello Everyone,
> I  have been using relion-5.0-beta-4-commit-267e0a and the Autopick 
> with topaz for a filament protein, but  I am facing issues with FOM 
> values of the micrographs. All of them have the same FOM value (i.e., 
> 0) so I can't adjust the FOM to get a good picking threshold for 
> Extraction. Any help would be appreciated.
>
> Regards
> Virajith
>
> _______________________________________________
> 3dem mailing list
> 3dem at ncmir.ucsd.edu
> https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem
-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://mail.ncmir.ucsd.edu/pipermail/3dem/attachments/20241107/e47094cf/attachment.html>