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<p>Hi Virajith,</p>
<p>This works slightly different from single particle topaz: you
need to specify the FOM threshold on the GUI to be used for the
extraction of entire filaments; you can no longer play with the
threshold afterwards in extraction, as you can for
single-particles. You can use the extra command -fp to get some
visual feedback for micrographs, which can help to decide the
threshold.<br>
</p>
<p>HTH,</p>
<p>Sjors</p>
<p><br>
</p>
<div class="moz-cite-prefix">On 07/11/2024 12:22, virajith cryo-em
wrote:<br>
</div>
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<p>
<br>
--<br>
</p>
<div dir="ltr">Hello Everyone,
<div>I have been using relion-5.0-beta-4-commit-267e0a and the
Autopick with topaz for a filament protein, but I am facing
issues with FOM values of the micrographs. All of them have
the same FOM value (i.e., 0) so I can't adjust the FOM to get
a good picking threshold for Extraction. Any help would be
appreciated.</div>
<div><br>
</div>
<div>Regards</div>
<div>Virajith</div>
</div>
<br>
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