[3dem] Calling Teamtomo: our CERES ICE-Shield isn't working properly

[Walsh, Richard Michael]

We have heard of several sites using the CERES Ice-Shield on their Aquilos with no issues, but we have a reoccurring problem, during the thinning stage and its effecting our lamella quality.


When using the CERES Ice-Shield in tandem with AutoTEM, we notice that each time the stage moves, the ice shield retracts and re-inserts, and thus during the thinning stage of the AutoTEM automation, the shield blocks reference image collection, thus resulting in a misplaced pattern and inconsistent lamella quality.


Link to Video For Reference: https://urldefense.com/v3/__https://drive.google.com/file/d/1dyQR57ZPr6SQv_AypQvH-uOSCqPkD9jp/view__;!!Mih3wA!E4y-pfw8EsMkATaLFu-CyxAq6fvhvv2ObUTsGRwM7Zuf9I7SZj3gejlAO1pKmek9JdeXQL0L8NxOyAI8Sr8M5sPNdEuM68CYxg$ 


So far neither Delmic nor ThermoFisher have any idea how to resolve this problem.


Any ideas would be greatly appreciated.


best regards,


Richard



Richard M. Walsh , Ph.D.

Managing Director
The Harvard Cryo-EM
Center for Structural Biology

Harvard Medical School
250 Longwood Avenue
SGM 102
Boston MA 02115

Phone: (617) 432-9042
Email:Richard_Walsh at hms.harvard.edu<mailto:Richard_Walsh at hms.harvard.edu>
Web: cryoem.hms.harvard.edu<https://urldefense.com/v3/__http://cryoem.hms.harvard.edu/__;!!Mih3wA!E4y-pfw8EsMkATaLFu-CyxAq6fvhvv2ObUTsGRwM7Zuf9I7SZj3gejlAO1pKmek9JdeXQL0L8NxOyAI8Sr8M5sPNdEuMOhc5rw$ >
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