[3dem] question about cisTEM

[Tim Grant]

Hi David,

Inverting in the sharpening panel will not propagate I am afraid.  There is
no easy way to do this, and there is no problem with refining the incorrect
handedness and changing it later, but I understand it can become annoying.
If you want to run refinements with the correct handedness, you could
import the inverted handedness model and rerun refinement against that.

Thanks,

Tim

On Tue, 16 Jan 2024 at 16:36, Morgan, David Gene <dagmorga at indiana.edu>
wrote:

> Hi,
>
> I'm making some reconstructions using cisTEM and need to invert the
> handedness of one of them in order to compare it to several others.  Does
> anyone happen to know whether this inversion "propagates" to the
> orientations used to generate the volume, and if not, how to invert them
> within the cisTEM system?
>
> Thanks in advance for any advice or suggestions.
> --
>     politics is more difficult than physics.
>                                              A. Einstein
>
>             David Gene Morgan
>         Electron Microscopy Center
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