<div dir="ltr">Hi David,<div><br></div><div>Inverting in the sharpening panel will not propagate I am afraid. There is no easy way to do this, and there is no problem with refining the incorrect handedness and changing it later, but I understand it can become annoying. If you want to run refinements with the correct handedness, you could import the inverted handedness model and rerun refinement against that.</div><div><br></div><div>Thanks,</div><div><br></div><div>Tim</div></div><br><div class="gmail_quote"><div dir="ltr" class="gmail_attr">On Tue, 16 Jan 2024 at 16:36, Morgan, David Gene <<a href="mailto:dagmorga@indiana.edu">dagmorga@indiana.edu</a>> wrote:<br></div><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex"><div class="msg4043683753493713272">
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Hi,</div>
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I'm making some reconstructions using cisTEM and need to invert the handedness of one of them in order to compare it to several others. Does anyone happen to know whether this inversion "propagates" to the orientations used to generate the volume, and if not,
how to invert them within the cisTEM system?</div>
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Thanks in advance for any advice or suggestions.<br>
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David Gene Morgan<br>
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