[3dem] Converting cryolo tomogram particle coordinates to Relion4/5 friendly ones

Zongli Li zongli.li at gmail.com
Thu Feb 29 11:11:33 PST 2024


Hi Willem,

The EMAN format from cryolo can be directly used in Relion. I have been
using this way for SPA all the time, but haven't used it in tomogram
particles. I assume they should be the same.

Best,

Zongli



On Thu, Feb 29, 2024 at 1:44 PM Willem Noteborn <willemnoteborn at outlook.com>
wrote:

> Dear list,
>
> I was wondering if anyone knows a way to convert cryolo picked tomogram
> coordinates (for instance the CBOX_3D or coords format) to a format that
> can be readily imported into Relion. I've been playing around with
> cryolo_boxmanager_tools.py but can't seem to find a solution. Maybe I'm
> missing something obvious.
>
> Any information on this would be highly appreciated!
>
> Kind regards,
> Willem
>
> Dr. Willem Noteborn
> CryoEM Scientist
>
> T: +31 (0)7 1527 1424
> w.e.m.noteborn at biology.leidenuniv.nl
> NeCEN at biology.leidenuniv.nl
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