<div dir="ltr"><div>Hi Willem,</div><div><br></div><div>The EMAN format from cryolo can be directly used in Relion. I have been using this way for SPA all the time, but haven't used it in tomogram particles. I assume they should be the same.</div><div><br></div><div>Best,</div><div><br></div><div>Zongli<br></div><div><br></div><div><br></div></div><br><div class="gmail_quote"><div dir="ltr" class="gmail_attr">On Thu, Feb 29, 2024 at 1:44 PM Willem Noteborn <<a href="mailto:willemnoteborn@outlook.com">willemnoteborn@outlook.com</a>> wrote:<br></div><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex"><div class="msg-8195834432432991172">
<div dir="ltr">
<div><span style="font-family:Calibri,Helvetica,sans-serif;font-size:12pt;color:rgb(0,0,0)">Dear list,</span></div>
<div><span style="font-family:Calibri,Helvetica,sans-serif;font-size:12pt;color:rgb(0,0,0)"><br>
</span></div>
<div><span style="font-family:Calibri,Helvetica,sans-serif;font-size:12pt;color:rgb(0,0,0)">I was wondering if anyone knows a way to convert cryolo picked tomogram coordinates (for instance the CBOX_3D or coords format) to a format that can be readily
imported into Relion. I've been playing around with cryolo_boxmanager_tools.py but can't seem to find a solution. Maybe I'm missing something obvious.</span></div>
<div><span style="font-family:Calibri,Helvetica,sans-serif;font-size:12pt;color:rgb(0,0,0)"><br>
</span></div>
<div><span style="font-family:Calibri,Helvetica,sans-serif;font-size:12pt;color:rgb(0,0,0)">Any information on this would be highly appreciated!</span></div>
<div><span style="font-family:Calibri,Helvetica,sans-serif;font-size:12pt;color:rgb(0,0,0)"><br>
</span></div>
<div><span style="font-family:Calibri,Helvetica,sans-serif;font-size:12pt;color:rgb(0,0,0)">Kind regards,</span></div>
<div><span style="font-family:Calibri,Helvetica,sans-serif;font-size:12pt;color:rgb(0,0,0)">Willem</span></div>
<div><span style="font-family:Calibri,Helvetica,sans-serif;font-size:12pt;color:rgb(0,0,0)"><br>
</span></div>
<div><span style="font-family:Calibri,Helvetica,sans-serif;font-size:12pt;color:rgb(0,0,0)">Dr. Willem Noteborn</span></div>
<div><span style="font-family:Calibri,Helvetica,sans-serif;font-size:12pt;color:rgb(0,0,0)">CryoEM Scientist</span></div>
<div><span style="font-family:Calibri,Helvetica,sans-serif;font-size:12pt;color:rgb(0,0,0)"> </span></div>
<div><span style="font-family:Calibri,Helvetica,sans-serif;font-size:12pt;color:rgb(0,0,0)">T: +31 (0)7 1527 1424</span></div>
<div><span style="font-family:Calibri,Helvetica,sans-serif;font-size:12pt;color:rgb(0,0,0)"><a href="mailto:w.e.m.noteborn@biology.leidenuniv.nl" target="_blank">w.e.m.noteborn@biology.leidenuniv.nl</a> </span></div>
<div><span style="font-family:Calibri,Helvetica,sans-serif;font-size:12pt;color:rgb(0,0,0)"><a href="mailto:NeCEN@biology.leidenuniv.nl" target="_blank">NeCEN@biology.leidenuniv.nl</a></span></div>
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