[3dem] Beam drift - Glacios G2

Daniel Asarnow asarnow at msg.ucsf.edu
Tue Feb 13 09:56:18 PST 2024


Hi Piotr,
If this is indeed simply a hysteresis issue, it could help to run a script
that programmatically conducts the same series of state changes you want
many times over. For example, I used to run a SerialEM script to cycle
view/focus/record 5-10 times with several seconds delay between each
switch, and optionally a pause for the user to center the beam.

Best,
-da

On Tue, Feb 13, 2024 at 1:52 AM Piotr Szwedziak <piotr.szwedziak at zmb.uzh.ch>
wrote:

> Dear All, We have realised that the electron beam of our Glacios G2
> (installed in December 2023) is not stable – for instance switching from
> imaging to diffraction and back causes the beam to return to a different
> position and continue
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> Dear All,
>
> We have realised that the electron beam of our Glacios G2 (installed in
> December 2023) is not stable – for instance switching from imaging to
> diffraction and back causes the beam to return to a different position and
> continue drifting for several minutes. More background information:
>
>
>
> -          switching on/off active electromagnetic field cancellation
> system doesn’t make a difference to the beam stability
>
> -          opening/closing the microscope enclosure, disturbing the
> electromagnetic field intentionally doesn’t make a difference to the beam
> stability
>
> -          a lower end EM (Talos 120), which is next door and in similar
> electromagnetic field environment, has a very stable beam that doesn’t
> drift
>
> -          the drift we observe on the Glacios G2 is particularly severe
> after changing microscope magnification/switching to diffraction
>
> -          the beam drift is unidirectional rather than in random
> orientations
>
> Therefore we believe the issue of the unstable beam is system- rather than
> environment-related. Has anyone experienced similar problems?
>
> Best and many thanks,
>
> Piotr
>
>
>
>
>
>
>
>
>
> --
> Piotr Szwedziak, PhD
> Center for Microscopy and Image Analysis
> University of Zurich
> Winterthurerstrasse 190
> CH-8057 Zurich
> Switzerland
>
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