<div dir="ltr"><div>Hi Piotr,</div><div>If this is indeed simply a hysteresis issue, it could help to run a script that programmatically conducts the same series of state changes you want many times over. For example, I used to run a SerialEM script to cycle view/focus/record 5-10 times with several seconds delay between each switch, and optionally a pause for the user to center the beam.<br></div><div><br></div><div>Best,</div><div>-da<br></div></div><br><div class="gmail_quote"><div dir="ltr" class="gmail_attr">On Tue, Feb 13, 2024 at 1:52 AM Piotr Szwedziak <<a href="mailto:piotr.szwedziak@zmb.uzh.ch">piotr.szwedziak@zmb.uzh.ch</a>> wrote:<br></div><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex"><div class="msg-6135693136354244470">
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Dear All, We have realised that the electron beam of our Glacios G2 (installed in December 2023) is not stable – for instance switching from imaging to diffraction and back causes the beam to return to a different position and continue
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<p class="MsoNormal">Dear All,<u></u><u></u></p>
<p class="MsoNormal">We have realised that the electron beam of our Glacios G2 (installed in December 2023) is not stable – for instance switching from imaging to diffraction and back causes the beam to return to a different position and continue drifting for
several minutes. More background information:<u></u><u></u></p>
<p class="MsoNormal"><u></u> <u></u></p>
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<u></u><span>-<span style="font:7pt "Times New Roman"">
</span></span><u></u>switching on/off active electromagnetic field cancellation system doesn’t make a difference to the beam stability<u></u><u></u></p>
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<u></u><span>-<span style="font:7pt "Times New Roman"">
</span></span><u></u>opening/closing the microscope enclosure, disturbing the electromagnetic field intentionally doesn’t make a difference to the beam stability<u></u><u></u></p>
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</span></span><u></u>a lower end EM (Talos 120), which is next door and in similar electromagnetic field environment, has a very stable beam that doesn’t drift
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<u></u><span>-<span style="font:7pt "Times New Roman"">
</span></span><u></u>the drift we observe on the Glacios G2 is particularly severe after changing microscope magnification/switching to diffraction<u></u><u></u></p>
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</span></span><u></u>the beam drift is unidirectional rather than in random orientations<u></u><u></u></p>
<p class="MsoNormal" style="margin-bottom:12pt">Therefore we believe the issue of the unstable beam is system- rather than environment-related. Has anyone experienced similar problems?
<u></u><u></u></p>
<p class="MsoNormal" style="margin-bottom:12pt">Best and many thanks,<u></u><u></u></p>
<p class="MsoNormal" style="margin-bottom:12pt">Piotr <u></u><u></u></p>
<p class="MsoNormal"><u></u> <u></u></p>
<p class="MsoNormal"><u></u> <u></u></p>
<p class="MsoNormal"><u></u> <u></u></p>
<p class="MsoNormal"><u></u> <u></u></p>
<p class="MsoNormal"><span>-- <br>
Piotr Szwedziak, PhD <br>
Center for Microscopy and Image Analysis <br>
University of Zurich <br>
Winterthurerstrasse 190 <br>
CH-8057 Zurich <br>
Switzerland <br>
<br>
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