[3dem] 3dem Digest, Vol 190, Issue 1

Thu Sep 14 10:31:47 PDT 2023

Dear Cornelia,

What is the product number of the ethane: propane mix, if you tracked it down?

Thank you,

Oleksiy

On 1. Jun 2023, at 15:27, Cazey, Cornelia <cornelia.cazey at cssb-hamburg.de<mailto:cornelia.cazey at cssb-hamburg.de>> wrote:

AMAZING!

I had just started a new email to the group, because Linde had come back to us with a pretty ridiculous offer in order to continue their supply. They offered to keep supplying the mixture under the proviso that every order contains at least 6x 10l bottles and they doubled the price to €900 (I assumed pre tax). They were willing to extend the current 12 months warranty to 36months, but nevertheless, we use max one bottle per year, so would still be pretty much wasting 2-3 bottles at the end of an order cycle (and that is assuming Linde won't scrap the equipment in the meantime due to absolutely no orders in the next 6 years with that hare-brained idea).

So these news are fantastic! Thanks for tracking them down!

Best wishes,

Cornelia

________________________________
From: 3dem-request at ncmir.ucsd.edu<mailto:3dem-request at ncmir.ucsd.edu>
To: "3dem" <3dem at ncmir.ucsd.edu>
Sent: Thursday, 1 June, 2023 15:03:48
Subject: 3dem Digest, Vol 190, Issue 1

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Today's Topics:

   1. Re: Calling all groups who use cryogen mix from Linde-Gas
      (Germany, maybe Austria, Switzerland?) for plunging (Dario)
   2. Postdoc position - computational tools for ?in situ? cryo-EM
      (Briggs, John)
   3. Group Leader postion in cryo EM / cryo ET @ Institut Pasteur,
      Paris (Michael  NILGES)

----------------------------------------------------------------------

Message: 1
Date: Thu, 1 Jun 2023 10:05:48 +0200
From: Dario <dariobrack at googlemail.com<mailto:dariobrack at googlemail.com>>
To: 3dem at ncmir.ucsd.edu<mailto:3dem at ncmir.ucsd.edu>
Subject: Re: [3dem] Calling all groups who use cryogen mix from
        Linde-Gas (Germany, maybe Austria, Switzerland?) for plunging
Message-ID:
        <CAHOoyBOLpbBBUf9YABm0sM5WQR9aaY9QbtN_jr43J-VBWPqPqw at mail.gmail.com<mailto:CAHOoyBOLpbBBUf9YABm0sM5WQR9aaY9QbtN_jr43J-VBWPqPqw at mail.gmail.com>>
Content-Type: text/plain; charset="utf-8"

Just for everyones information:
After contacting basically each gas supplier in Germany, I finally found
one that can produce and deliver the liquid Ethane:propane mixture (at
least in Germany)

Nippon-gases
https://urldefense.com/v3/__https://nippongases.com/__;!!Mih3wA!EWcu8XZh3yPCwu6EsK7-hEtzSFtvLJcLuVmsBcMPfQi6BJeV1_kJpKhkAHqLrcZJSuWkePAPJ4sLNBH6RLdij0fTGA$

Cornelia Cazey <cornelia.cazey at cssb-hamburg.de<mailto:cornelia.cazey at cssb-hamburg.de>> schrieb am Di., 25. Apr.
2023, 13:50:

Hi Oliver,

 Thanks a lot for the info. But why do they only supply in 50l cylinders?
 Or rather why order a 50l cylinder? Economic reasons?

 Thanks!

 Cornelia
 On 25 Apr 2023, at 13:40, Oliver Hofnagel <
 oliver.hofnagel at mpi-dortmund.mpg.de<mailto:oliver.hofnagel at mpi-dortmund.mpg.de>> wrote:

 Dear Cornelia,

 here our supplier for  37% ethane / 63% propane 50l cylinder cryogen mix
 in Germany:

 *AIR LIQUIDE Deutschland GmbH Kunden-Service-Center Spezialgase
 Bataverstra?e 47 47809 Krefeld Telefon: (02151) 954-254 **Email :
 spezialgase.ald at airliquide.com<mailto:spezialgase.ald at airliquide.com> <spezialgase.ald at airliquide.com<mailto:spezialgase.ald at airliquide.com>>*

 Its important to order a 50l cylinder, not a 10l cylinder !

 Greetings,

 Oliver

 Dr. Oliver Hofnagel
 Max-Planck-Institute of Molecular Physiology
 Electron Microscopy
 Otto-Hahn-Str. 11
 44227 Dortmund
 Germany

 ------------------------------
 *Von:* 3dem <3dem-bounces at ncmir.ucsd.edu<mailto:3dem-bounces at ncmir.ucsd.edu>> im Auftrag von Michael Elbaum <
 michael.elbaum at weizmann.ac.il<mailto:michael.elbaum at weizmann.ac.il>>
 *Gesendet:* Donnerstag, 20. April 2023 16:29:00
 *An:* r.i.koning at lumc.nl<mailto:r.i.koning at lumc.nl>; cornelia.cazey at cssb-hamburg.de<mailto:cornelia.cazey at cssb-hamburg.de>;
 3dem at ncmir.ucsd.edu<mailto:3dem at ncmir.ucsd.edu>
 *Betreff:* Re: [3dem] Calling all groups who use cryogen mix from
 Linde-Gas (Germany, maybe Austria, Switzerland?) for plunging

 Hi.  would it be possible to use two pure tanks and a mixing regulator? I
 think such regulators are used for welding.

 Michael
 ------------------------------
 *From:* 3dem <3dem-bounces at ncmir.ucsd.edu<mailto:3dem-bounces at ncmir.ucsd.edu>> on behalf of
 r.i.koning at lumc.nl<mailto:r.i.koning at lumc.nl> <r.i.koning at lumc.nl<mailto:r.i.koning at lumc.nl>>
 *Sent:* Thursday, April 20, 2023 5:15:10 PM
 *To:* cornelia.cazey at cssb-hamburg.de<mailto:cornelia.cazey at cssb-hamburg.de>; 3dem at ncmir.ucsd.edu<mailto:3dem at ncmir.ucsd.edu>
 *Subject:* Re: [3dem] Calling all groups who use cryogen mix from
 Linde-Gas (Germany, maybe Austria, Switzerland?) for plunging

 Dear Cornelia,

 Our most recent order of ethane/propane also did not come through from
 Line because of the same reason. We will have to do with ethane only for
 now but I prefer the mixture for obvious reasons, though we do manage with
 ethane only. We did not look for nor found another vendor.

 Best regards,

 Roman

 *Dr. R.I.Koning*

 Leiden University Medical Center, Cell and Chemical Biology, Electron
 Microscopy

 Postal Zone S1-P, P.O.Box 9600, 2300 RC, Leiden, The Netherlands

 *visiting address.* Einthovenweg 20, Building 2, zone S1-P, room R-90-21

 *tel.* (31) 71 526 9296 *fax.* (+31) 71 526 8270 *mail.*
 r.i.koning at lumc.nl<mailto:r.i.koning at lumc.nl> *web. *Electron Microscopy Facility
 <https://urldefense.com/v3/__https://ccb.lumc.nl/electron-microscopy-198__;!!Mih3wA!GxgfkasxXThNdN_k1a9iqn0d9uls-PLaHnrrJAIhFXNITn6ehlzCx_U6V-rQFPvgm8pSGK1CJ1Vd55FxuUK3uvc$>

 *From:* 3dem <3dem-bounces at ncmir.ucsd.edu<mailto:3dem-bounces at ncmir.ucsd.edu>> *On Behalf Of *Cazey, Cornelia
 *Sent:* donderdag 20 april 2023 15:52
 *To:* 3dem at ncmir.ucsd.edu<mailto:3dem at ncmir.ucsd.edu>
 *Subject:* [3dem] Calling all groups who use cryogen mix from Linde-Gas
 (Germany, maybe Austria, Switzerland?) for plunging

 Dear list participants,

 Our lab is in Germany, and this is a very particular question relating to
 a German supplier, so apologies for addressing the entire list, but it
 seemed the easiest way to find groups who may be able to help.

 We recently learned that Linde-Gas, our supplier for the cryogen mix we
 use in our facility (37% ethane, rest propane) is no longer able to supply
 this mixture due to the overall difficulties in the production, age and
 poor state of their necessary equipment.

 I spoke with the supplier yesterday and while they would like to continue
 to offer the mix, they feel there is not enough market to make it feasible.
 I am now looking for info from groups who are using a cryogen mix rather
 than pure ethane for plunging (I am not sure, but relatively convinced
 that the process would be the same for any kind of mixture, so I guess the
 exact percentages won't matter too much) and have previously sourced
 this from Linde-Gas, and might be or have already been affected by this
 decision. If you have been affected, did you find a solution, a different
 supplier or any other alternative you are willing to share? Or if you
 didn't yet know, how much would this news affect your ability to work, and
 would you be interested to try and convince Linde-Gas to keep going? I
 understand the production line has already been shut down, but could be
 revived with enough interest, however, it may also come with a price
 increase (what doesn't).

 Any help would be greatly appreciated, whether info on alternatives or
 willingness to push for the supply. I am happy to collect replies to send
 to the Linde-Gas representative in bulk or, of course, for those who prefer
 to contact him directly, do so themselves and increase the pressure to keep
 going/restart production.

 Thanks a lot for your help!

 Best wishes,

 Cornelia Cazey

 Cornelia Cazey (She/Her)

 Cryo-EM Facility

 Centre for Structural Systems Biology (CSSB)
 c/o DESY, Building 15
 Notkestr. 85
 22607 Hamburg
 Germany

 phone: +49-(0)40-8998-87706
 email:*cornelia.cazey at cssb-hamburg.de<mailto:cornelia.cazey at cssb-hamburg.de> <cornelia.cazey at cssb-hamburg.de<mailto:cornelia.cazey at cssb-hamburg.de>>*

 *"If you don't know it's impossible, it's easier to do"* -Neil Gaiman

 Sometimes I may send emails outside of core working hours. I do not
 expect you to read, or respond outside of your own working hours!

 _______________________________________________
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Message: 2
Date: Thu, 1 Jun 2023 12:13:54 +0000
From: "Briggs, John" <briggs at biochem.mpg.de<mailto:briggs at biochem.mpg.de>>
To: "3dem at ncmir.ucsd.edu<mailto:3dem at ncmir.ucsd.edu>" <3dem at ncmir.ucsd.edu<mailto:3dem at ncmir.ucsd.edu>>
Subject: [3dem] Postdoc position - computational tools for ?in situ?
        cryo-EM
Message-ID: <500F7DD0-20CD-49F9-B489-5CB4C18A5B47 at biochem.mpg.de<mailto:500F7DD0-20CD-49F9-B489-5CB4C18A5B47 at biochem.mpg.de>>
Content-Type: text/plain; charset="utf-8"

Dear All,

We have a postdoc position available to develop theoretical frameworks and computational tools for image analysis of ?in situ? cryo-electron microscopy data. You should have a PhD in a relevant subject (computational sciences, physics, biophysics or similar) and a strong interest in the theory and methods of cryo-electron microscopy and image analysis by classical or machine learning approaches.

In the lab we apply cryo-electron microscopy and image processing to determine the structures and distributions of protein complexes ?in situ? from within viruses, cells and organisms. The Max Planck Institute of Biochemistry has outstanding facilities for cryo-electron tomography, microscopy, and image analysis. The successful candidate will join a large, vibrant community developing and applying these methods.

Applications by 23rd June via the link on this page: https://urldefense.com/v3/__https://www.mpg.de/20379064/postdoc-position-cell-and-virus-structure__;!!Mih3wA!DkVqxEpeWDpJDwoGB1bUizx9xbiFbQEgV7BhJRhNOSNVEJ91-gDAB4h0TL_wqF26MfkQHnpraoeNu7fBUyUeozc$

You can contact me with informal enquiries.

John Briggs
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Message: 3
Date: Thu, 1 Jun 2023 13:03:44 +0000
From: "Michael  NILGES" <michael.nilges at pasteur.fr<mailto:michael.nilges at pasteur.fr>>
To: "3dem at ncmir.ucsd.edu<mailto:3dem at ncmir.ucsd.edu>" <3dem at ncmir.ucsd.edu<mailto:3dem at ncmir.ucsd.edu>>
Subject: [3dem] Group Leader postion in cryo EM / cryo ET @ Institut
        Pasteur,        Paris
Message-ID: <BF3808F4-498A-44EE-8788-3D9A7C754962 at pasteur.fr<mailto:BF3808F4-498A-44EE-8788-3D9A7C754962 at pasteur.fr>>
Content-Type: text/plain; charset="us-ascii"

Dear 3DEM community,

The Structural Biology and Chemistry Department at Institut Pasteur has an opening for a junior / mid career group leader in electron microscopy.
The deadline for applications is July 15, 2023.

<https://urldefense.com/v3/__https://research.pasteur.fr/en/call/junior-mid-career-group-leader-position-in-structural-biology-cryo-electron-microscopy-tomography/__;!!Mih3wA!ApBbafdgZUYubegPYjaYSoE5QtF_bGMDjjFfFuZL5ROfksfaE43TOrNtOIjQeVHTXv-UDjTU8syV10OueFiyAGbwJX2grw$ >

[cid:3433ad4b-4074-41d4-8515-c550cc1620ab at corp.pasteur.fr]

<https://urldefense.com/v3/__https://research.pasteur.fr/en/call/junior-mid-career-group-leader-position-in-structural-biology-cryo-electron-microscopy-tomography/__;!!Mih3wA!ApBbafdgZUYubegPYjaYSoE5QtF_bGMDjjFfFuZL5ROfksfaE43TOrNtOIjQeVHTXv-UDjTU8syV10OueFiyAGbwJX2grw$ >

Junior/Mid-career Group Leader position in Structural Biology/Cryo-electron microscopy & tomography - Research<https://urldefense.com/v3/__https://research.pasteur.fr/en/call/junior-mid-career-group-leader-position-in-structural-biology-cryo-electron-microscopy-tomography/__;!!Mih3wA!ApBbafdgZUYubegPYjaYSoE5QtF_bGMDjjFfFuZL5ROfksfaE43TOrNtOIjQeVHTXv-UDjTU8syV10OueFiyAGbwJX2grw$ >
research.pasteur.fr<https://urldefense.com/v3/__http://research.pasteur.fr__;!!Mih3wA!CekSS3f-o_zH87Hry39JeJNu9GJ55szbmuGF28SoMt9ybgbfOeqb3PcbQlLRs0WAzYF4eWXUthIOu-huxuAR3p8GGzIrOq7bxA$ ><https://urldefense.com/v3/__https://research.pasteur.fr/en/call/junior-mid-career-group-leader-position-in-structural-biology-cryo-electron-microscopy-tomography/__;!!Mih3wA!ApBbafdgZUYubegPYjaYSoE5QtF_bGMDjjFfFuZL5ROfksfaE43TOrNtOIjQeVHTXv-UDjTU8syV10OueFiyAGbwJX2grw$ >

Best regards

Michael

===============
Prof. Michael Nilges
Head of Structural Bioinformatics Unit
Vice president of Technology
Institut Pasteur, Paris
Tel: +33 1 45 68 82 30
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