[3dem] Choosing some micrographs from a large dataset

[Gabriel Abraham Frank]

Hi Esther,

The way I do such tasks is to down-sample all the micrographs and write them as PNG files.
I then move the down-sampled PNG files along with the star file to a local machine where I can conveniently select the relevant ones and put them in a separate directory.
I then run a script deleting the micrographs not selected from the star file.

Let me know if you are interested, and I will send you my scripts.

Best,
Gaby

_
Dr. Gabriel A. Frank
Structural Biology & Cryo-EM
Department of Life Sciences and NIBN
Ben-Gurion University of the Negev
Office: 972-8-642-8621
Lab: 972-8-642-8852
Building 41/128
https://urldefense.com/v3/__https://www.frank-cryoem.com/__;!!Mih3wA!CZudji9g6GtoABA-NC-Zf1zoDpASl59A3wkCjNC5kl_ZTPdD3X18ASv1Rv79yF3dp4nyc1JQjUev_PPdnfc$ 



From: 3dem <3dem-bounces at ncmir.ucsd.edu> On Behalf Of Bullitt, Esther
Sent: Friday, October 6, 2023 12:44 AM
To: 3DEM List (3dem at ncmir.ucsd.edu) <3dem at ncmir.ucsd.edu>
Subject: [3dem] Choosing some micrographs from a large dataset

Hi,

I would like to look in RELION at all of the micrographs in my dataset (~3,000) and Select the rare ones that have a specific morphology of interest.

Do I really need to load all 3K images at the same time in 'Subset Selection’?
Because I don’t see how to start at, say, micrograph 251 after first loading the first 250 images and making that selection.

Probably not relevant, but I can see what I need at 0.2x.

Thanks,
Esther

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