<html xmlns:v="urn:schemas-microsoft-com:vml" xmlns:o="urn:schemas-microsoft-com:office:office" xmlns:w="urn:schemas-microsoft-com:office:word" xmlns:dt="uuid:C2F41010-65B3-11d1-A29F-00AA00C14882" xmlns:m="http://schemas.microsoft.com/office/2004/12/omml" xmlns="http://www.w3.org/TR/REC-html40">
<head>
<meta http-equiv="Content-Type" content="text/html; charset=utf-8">
<meta name="Generator" content="Microsoft Word 15 (filtered medium)">
<style><!--
/* Font Definitions */
@font-face
{font-family:Helvetica;
panose-1:2 11 6 4 2 2 2 2 2 4;}
@font-face
{font-family:"Cambria Math";
panose-1:2 4 5 3 5 4 6 3 2 4;}
@font-face
{font-family:Calibri;
panose-1:2 15 5 2 2 2 4 3 2 4;}
/* Style Definitions */
p.MsoNormal, li.MsoNormal, div.MsoNormal
{margin:0cm;
font-size:11.0pt;
font-family:"Calibri",sans-serif;}
span.EmailStyle19
{mso-style-type:personal-reply;
font-family:"Calibri",sans-serif;
color:windowtext;}
.MsoChpDefault
{mso-style-type:export-only;
font-size:10.0pt;
mso-ligatures:none;}
@page WordSection1
{size:612.0pt 792.0pt;
margin:72.0pt 90.0pt 72.0pt 90.0pt;}
div.WordSection1
{page:WordSection1;}
--></style><!--[if gte mso 9]><xml>
<o:shapedefaults v:ext="edit" spidmax="1026" />
</xml><![endif]--><!--[if gte mso 9]><xml>
<o:shapelayout v:ext="edit">
<o:idmap v:ext="edit" data="1" />
</o:shapelayout></xml><![endif]-->
</head>
<body lang="EN-US" link="#0563C1" vlink="#954F72" style="word-wrap:break-word">
<div class="WordSection1">
<p class="MsoNormal">Hi Esther,<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">The way I do such tasks is to down-sample all the micrographs and write them as PNG files.<o:p></o:p></p>
<p class="MsoNormal">I then move the down-sampled PNG files along with the star file to a local machine where I can conveniently select the relevant ones and put them in a separate directory.<o:p></o:p></p>
<p class="MsoNormal">I then run a script deleting the micrographs not selected from the star file.<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Let me know if you are interested, and I will send you my scripts.<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Best,<o:p></o:p></p>
<p class="MsoNormal">Gaby<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><u><span style="color:#A6A6A6">_ </span></u><span style="font-family:"Arial",sans-serif"><o:p></o:p></span></p>
<p class="MsoNormal"><b><span style="color:#002060">Dr. Gabriel A. Frank </span></b><o:p></o:p></p>
<p class="MsoNormal"><b><span style="color:#002060">Structural Biology & Cryo-EM</span></b><span lang="HE" dir="RTL" style="font-family:"Arial",sans-serif"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:#002060">Department of Life Sciences and NIBN</span><o:p></o:p></p>
<p class="MsoNormal"><span style="color:#002060">Ben-Gurion University of the Negev</span><o:p></o:p></p>
<p class="MsoNormal"><span style="color:#002060">Office: 972-8-642-8621</span><o:p></o:p></p>
<p class="MsoNormal"><span style="color:#002060">Lab: 972-8-642-8852</span><o:p></o:p></p>
<p class="MsoNormal"><span style="color:#002060">Building 41/128 </span><o:p></o:p></p>
<p class="MsoNormal"><span style="color:black"><a href="https://urldefense.com/v3/__https://www.frank-cryoem.com/__;!!Mih3wA!CZudji9g6GtoABA-NC-Zf1zoDpASl59A3wkCjNC5kl_ZTPdD3X18ASv1Rv79yF3dp4nyc1JQjUev_PPdnfc$"><span style="color:#0563C1">https://www.frank-cryoem.com/</span></a></span><o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<div>
<div style="border:none;border-top:solid #E1E1E1 1.0pt;padding:3.0pt 0cm 0cm 0cm">
<p class="MsoNormal"><b>From:</b> 3dem <3dem-bounces@ncmir.ucsd.edu> <b>On Behalf Of
</b>Bullitt, Esther<br>
<b>Sent:</b> Friday, October 6, 2023 12:44 AM<br>
<b>To:</b> 3DEM List (3dem@ncmir.ucsd.edu) <3dem@ncmir.ucsd.edu><br>
<b>Subject:</b> [3dem] Choosing some micrographs from a large dataset<o:p></o:p></p>
</div>
</div>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Hi, <o:p></o:p></p>
<div>
<p class="MsoNormal"><o:p> </o:p></p>
</div>
<div>
<p class="MsoNormal">I would like to look in RELION at all of the micrographs in my dataset (~3,000) and Select the rare ones that have a specific morphology of interest. <o:p></o:p></p>
</div>
<div>
<p class="MsoNormal"><o:p> </o:p></p>
</div>
<div>
<p class="MsoNormal">Do I really need to load all 3K images at the same time in 'Subset Selection’? <o:p></o:p></p>
</div>
<div>
<p class="MsoNormal">Because I don’t see how to start at, say, micrograph 251 after first loading the first 250 images and making that selection.<o:p></o:p></p>
</div>
<div>
<p class="MsoNormal"><o:p> </o:p></p>
</div>
<div>
<p class="MsoNormal">Probably not relevant, but I can see what I need at 0.2x.<o:p></o:p></p>
</div>
<div>
<p class="MsoNormal"><o:p> </o:p></p>
</div>
<div>
<p class="MsoNormal">Thanks,<o:p></o:p></p>
</div>
<div>
<p class="MsoNormal">Esther<o:p></o:p></p>
</div>
<div>
<div>
<div>
<div>
<div>
<div>
<div>
<div>
<div>
<div>
<div>
<div>
<div>
<div>
<p class="MsoNormal"><span style="font-size:9.0pt;font-family:"Helvetica",sans-serif;color:black"><o:p> </o:p></span></p>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
</body>
</html>