[3dem] Cryo-FIB Scientist position at UTSouthwestern Medical Center CEMF

Daniel Stoddard Daniel.Stoddard at UTSouthwestern.edu
Wed Mar 15 06:11:30 PDT 2023


Hello 3dem folks,


UTSouthwestern Medical Center in Dallas is seeking a talented and motivated staff scientist with experience in cryo-Focused Ion Beam (Cryo-FIB) technologies and cryo-electron tomography to join our growing team at the UT Southwestern Medical Center Cryo-Electron Microscopy Facility (CEMF). The CEMF contains two Aquilos II cryo-FIB mills (ThermoFisher Scientific) with integrated fluorescence light microscopes and a stand-alone Stellaris 5 (Leica) confocal fluorescence microscope in environmentally controlled facilities, Leica ICE High Pressure Freezer, wet-lab space for cell culture and sample preparation, and significant computational infrastructure for operational support. These facilities work in conjunction with our two Titan Krios, Talos Arctica and Glacios electron microscopes, all equipped with direct electron detectors. The CEMF is used by scientists across the biophysical and biological spectra to solve problems spanning signaling transduction, cell biology, neuroscience and immunology.



Significant cryo-FIB experience from Academia or Industry will be considered, PhD degree and >1 year experience working in a cryoEM core facility/Cryo-FIB is preferred. Title and salary will be commensurate with experience. The ideal candidate will have excellent communication skills to interface with internal and external customers as well as vendors regarding the equipment and operation of the facility.



Responsibilities include:



  *   Milling/Processing samples as a core service to achieve the highest possible quality lamella for subsequent cryo-EM imaging.
  *   Consulting with structural biologists along with the CEMF director regarding FIB projects undertaken at the core.
  *   Interfacing and collaborating with the UTSW Structural Biology Lab (SBL) which offers preliminary screening, data collection, and reconstruction of cryoEM/ET samples.
  *   Implementing innovative FIB milling processes/data collection strategies that will benefit our user community.
  *   maintaining accurate logs for microscope performance/usage and managing the usage schedule for the instruments via ilab.
  *   Interface with in-house cryoEM experts, IT specialists, and field service engineers to manage and ensure optimal performance of an Aquilos II system



UTSouthwestern is consistently rated one of the nation’s best Employers. This position is full time and includes full benefits, retirement/pension, and no-cost BCBS health insurance coverage for the individual employee.

Best,

-Daniel

Daniel J. Stoddard
Assistant Professor, Department of Biophysics
CryoEM Facility (CEMF) Director
University of Texas Southwestern Medical Center
6001 Harry Hines blvd, Dallas, TX 75390

________________________________

UT Southwestern

Medical Center

The future of medicine, today.
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