[3dem] Falcon 4(i) issues

[Andrea Nans]

Hi Julika,

This positive defocus issue in LM EFTEM started immediately after updating
our Krios system with Selectris/Falcon 4 i to TEM software 3.15.1 with FFI.
I understand the Thermo engineer plans to "change a value in Temoptics
maps," next week in order to fix this problem. It seems to be only a
problem in EFTEM mode.

I'm not sure if this is the same as adjusting a focus calibration value or
whether it will impact use of the Ceta. I'll let you know what happens.

Best,

Andrea

On Thu, Jun 15, 2023 at 2:29 PM Radecke, Julika (DLSLtd,RAL,LSCI) <
julika.radecke at diamond.ac.uk> wrote:

> Dear all,
>
> I have a question and a note for Falcon 4(i) users.
>
> The question is, we have 2 Krioses at Harwell Campus equipped with a
> Falcon 4i (and SelectrisX) and for both of them in order to get a defocus
> value at gridsquare magnification in EPU and possibly Tomo (?, I can't test
> this anymore on our system) we have to put the equivalent positive value
> in. Has anyone else encountered this problem on a Krios? We have a few
> ideas i.e. a bug, or EFTEM or some calibration related problems. We
> circumvented the problem on one system now by changing a Focus calibration
> value from positive to negative which however inverts the defocus input
> values on the Ceta. The problem does not occur on our Glacioses equipped
> with a Falcon 4i. I would be interested to know if you do or do not see the
> problem on either a 300 or 200 kV system, and also if the problem occurs in
> serialEM? The problem does not occur on our K3 systems.
>
> And a note for everyone using the Falcon 4(i) *not* on the latest TEM
> server version, if traffic light is running, multishot collections are
> significantly slower (i.e. 370 shots per hour versus 515 when traffic light
> is switched off), the bug has been fixed in the latest TEM server version.
>
> Best,
> Julika
>
> Dr. Julika Radecke
>
> EM Scientist
>
> Electron Bio-Imaging Centre (eBIC)
>
> Diamond Light Source
>
> Harwell Science & Innovation Research Campus
>
> OX11 0DE
>
>
> 01235 778931
>
>
>
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