<div dir="ltr">Hi Julika,<br><div><br></div><div>This positive defocus issue in LM EFTEM started immediately after updating our Krios system with Selectris/Falcon 4 i to TEM software 3.15.1 with FFI. I understand the Thermo engineer plans to "<span style="color:rgb(36,36,36);font-family:Calibri,sans-serif;font-size:14.6667px">change a value in Temoptics maps," next week in order to fix this problem. </span><span style="color:rgb(36,36,36);font-family:Calibri,sans-serif;font-size:14.6667px">It seems to be only a problem in EFTEM mode.</span><span style="color:rgb(36,36,36);font-family:Calibri,sans-serif;font-size:14.6667px"> </span></div><div><span style="color:rgb(36,36,36);font-family:Calibri,sans-serif;font-size:14.6667px"><br></span></div><div><span style="color:rgb(36,36,36);font-family:Calibri,sans-serif;font-size:14.6667px">I'm not sure if this is the same as adjusting a focus calibration value or whether it will impact use of the Ceta. I'll let you know what happens.</span><br></div><div><span style="color:rgb(36,36,36);font-family:Calibri,sans-serif;font-size:14.6667px"><br></span></div><div><span style="color:rgb(36,36,36);font-family:Calibri,sans-serif;font-size:14.6667px">Best,</span></div><div><span style="color:rgb(36,36,36);font-family:Calibri,sans-serif;font-size:14.6667px"><br></span></div><div><span style="color:rgb(36,36,36);font-family:Calibri,sans-serif;font-size:14.6667px">Andrea</span></div></div><br><div class="gmail_quote"><div dir="ltr" class="gmail_attr">On Thu, Jun 15, 2023 at 2:29 PM Radecke, Julika (DLSLtd,RAL,LSCI) <<a href="mailto:julika.radecke@diamond.ac.uk">julika.radecke@diamond.ac.uk</a>> wrote:<br></div><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex"><div class="msg5874771208429324219">
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Dear all,</div>
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I have a question and a note for Falcon 4(i) users.</div>
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The question is, we have 2 Krioses at Harwell Campus equipped with a Falcon 4i (and SelectrisX) and for both of them in order to get a defocus value at gridsquare magnification in EPU and possibly Tomo (?, I can't test this anymore on our system) we have to
put the equivalent positive value in. Has anyone else encountered this problem on a Krios? We have a few ideas i.e. a bug, or EFTEM or some calibration related problems. We circumvented the problem on one system now by changing a Focus calibration value from
positive to negative which however inverts the defocus input values on the Ceta. The problem does not occur on our Glacioses equipped with a Falcon 4i. I would be interested to know if you do or do not see the problem on either a 300 or 200 kV system, and
also if the problem occurs in serialEM? The problem does not occur on our K3 systems.</div>
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And a note for everyone using the Falcon 4(i) <b>not</b> on the latest TEM server version, if traffic light is running, multishot collections are significantly slower (i.e. 370 shots per hour versus 515 when traffic light is switched off), the bug has been
fixed in the latest TEM server version.</div>
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Best,</div>
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Julika</div>
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<p style="margin-top:0px;margin-bottom:0px">Dr. Julika Radecke</p>
<p style="margin-top:0px;margin-bottom:0px">EM Scientist<br>
</p>
<p style="margin-top:0px;margin-bottom:0px">Electron Bio-Imaging Centre (eBIC)</p>
<p style="margin-top:0px;margin-bottom:0px">Diamond Light Source</p>
<p style="margin-top:0px;margin-bottom:0px">Harwell Science & Innovation Research Campus</p>
<p style="margin-top:0px;margin-bottom:0px">OX11 0DE</p>
<p style="margin-top:0px;margin-bottom:0px"><br>
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<p style="margin-top:0px;margin-bottom:0px">01235 778931<br>
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