[3dem] Going from Amira filament picks to subtomogram averaging

Matthew Chang changmlw at umich.edu
Wed Jul 5 07:30:41 PDT 2023


Hello all,

I segmented filaments in Amira manually. I want to use that segmentation to
extract coordinates with tilt and psi angle priors to do subtomogram
reconstruction in Warp and subtomogram averaging in relion (using helical
processing). Does anyone know if this is possible?

Any help would be appreciated!

Thank you,
Matthew
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