[3dem] Advice for grid storage for a new lab

[Benjamin Apker]

Dear Abris,

We might have a slight bias, but are happy to contribute and assist the
community.

Our Cryo-EM and CrystallographyPuck systems are now in hundreds of labs
around the world, so we are familiar with your situation and the
considerations around transitioning to a puck-based system, as well as
setting up a new Cryo-EM lab and optimizing workflows.

For the sample storage, we do offer demos of the puck system, if you
are interested in some hands-on experience.

There are a number of other current developments in new small
equipment/starter kits
<https://urldefense.com/v3/__https://www.mitegen.com/product-category/cryo-em/cryo-em-starter-kits/__;!!Mih3wA!AOmB8Dzz4sVvDe7usch_phMoGmDWZ8697cZrKEzAyA0uLze8uhGIl5XGzBuYMfkRZdgYwhYlb-yfZFIwUmjXSLw1nUE9$ >
 and consumables
<https://urldefense.com/v3/__https://www.mitegen.com/product-category/cryo-em/grid-boxes-and-accessories/__;!!Mih3wA!AOmB8Dzz4sVvDe7usch_phMoGmDWZ8697cZrKEzAyA0uLze8uhGIl5XGzBuYMfkRZdgYwhYlb-yfZFIwUmjXSILjm0LM$ >,
so we would be happy to make ourselves available for a call to answer
questions about best practices and explore options.

Here are a few highlights that might be helpful new tools, including:

• Plunge Cooler & Temperature-controlled Cryostat
<https://urldefense.com/v3/__https://www.mitegen.com/product/manual-plunger-cooler-and-precision-cryostat/__;!!Mih3wA!AOmB8Dzz4sVvDe7usch_phMoGmDWZ8697cZrKEzAyA0uLze8uhGIl5XGzBuYMfkRZdgYwhYlb-yfZFIwUmjXSCzdP91b$ >
(licensed
from MRC LMB & Dr. Christopher Russo)
• Hybrid Cryo Grid Boxes
<https://urldefense.com/v3/__https://www.mitegen.com/product/hybrid-cryo-em-grid-boxes/__;!!Mih3wA!AOmB8Dzz4sVvDe7usch_phMoGmDWZ8697cZrKEzAyA0uLze8uhGIl5XGzBuYMfkRZdgYwhYlb-yfZFIwUmjXSIY0_8qJ$ >
• Grid Clipping Station <https://urldefense.com/v3/__https://www.mitegen.com/product/clipping-station/__;!!Mih3wA!AOmB8Dzz4sVvDe7usch_phMoGmDWZ8697cZrKEzAyA0uLze8uhGIl5XGzBuYMfkRZdgYwhYlb-yfZFIwUmjXSN6GGgqN$ >


and a New Lab Program <https://urldefense.com/v3/__https://www.mitegen.com/learn/new-lab/__;!!Mih3wA!AOmB8Dzz4sVvDe7usch_phMoGmDWZ8697cZrKEzAyA0uLze8uhGIl5XGzBuYMfkRZdgYwhYlb-yfZFIwUmjXSGsBMUC8$ > (to help
labs get the most from their setup funds).


Simplifying and improving sample preparation & storage in Cryo-EM is a
focus for us, so we are excited to be part of the discussion!

Best wishes,
Ben

On Wed, Jul 5, 2023 at 6:30 AM Abris Bendes <Abris.Bendes at abo.fi> wrote:

> Dear all,
>
>
> We are setting up a cryoEM sample preparation lab (mostly for SPA) from
> scratch, and still missing some smaller equipment and consumables. I would
> like to ask for your input on what is regarded as best practices for a
> smaller starting-out lab. What is currently the best option for boxes and
> storage of non-clipped grids? I have seen the emergence of twist-top grid
> boxes (eg from SWISSCI) and pucks (eg MiTeGen Gen 2), but have no
> experience with them. Any suggestions or other tips, not just for storage
> options, for a beginner lab would be most welcome.
>
>
> Best regards,
>
> Abris
>
>
> _______________________________________________
>
> *Ábris Á. Bendes, PhD*
>
> InFLAMES Flagship postdoctoral researcher
>
> Salminen lab / SBL / Åbo Akademi University
>
> Jalkanen and Hollmén lab / MediCity / University of Turku
>
> _______________________________________________
> 3dem mailing list
> 3dem at ncmir.ucsd.edu
> https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem
>


-- 
Benjamin Apker
Chief Executive Officer
MiTeGen

95 Brown Road
MS 1034, Suite 124
Ithaca, NY 14850
office: +1-607-266-8877
fax:    +1-607-697-0400
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