[3dem] Dry nitrogen generator that supports Aquilos cryo FIB

[Sasha Dickinson]

Hi Xinran,

We also do not have house N2 gas so I tried a few different N2 sources and
found that the best / most reasonable one was a standard ~5000 cubic feet
tank at 350 PSI (as opposed to the typical 22 PSI). This tank usually lasts
us >1 week on our Aquilos 2, so I usually have it replaced weekly like the
ones for the cryoTEMs.

Very best,
Sasha

On Wed, Jul 5, 2023 at 11:33 AM Liu, Xinran <xinran.liu at yale.edu> wrote:

> Dear Colleagues,
>
>
>
> I am looking for help to identify a dry N2 generator that can work with
> the Aquilos II cryo FIB (Thermo Fisher).
>
>
>
> It has become a concern that the Aquilos milling operation requires a huge
> volume of dry nitrogen that makes use of typical cylinders not practical.
> Because our building does not have a N2 supply source, we will have to
> purchase and install one that works with the Aquilos. Our multiple attempts
> to reach out to Thermo Fisher have not generated any response so far. The
> following are requirements for the generator, if someone here knows one or
> uses one that meet the criteria, can you share the information? much
> appreciated.
>
>
>
> *N2 at connection of the microscope*
>
> 0.5x105 Pa (7.25 psi, 0.5 bar) to 0.8 x105 Pa (11.6 psi, 0.8 bar)
>
> Flow rate is 30 liters/min
>
> The water content must be less than 10 ppm
>
>
>
> Xinran Nick Liu, M.D. & Ph.D.
>
> Director, Center for Cellular & Molecular Imaging
>
> Electron Microscopy Core Facility
>
> Senior Research Scientist in Cell Biology
>
> Yale University School of Medicine
>
> Office Phone: 203.785.4050
>
> Lab Phone: 203.785.5390
>
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