[3dem] [+externe Mail+] Re: Dry nitrogen generator that supports Aquilos cryo FIB

[Cheng, Tat Cheung]

Hi Xinran,

We also don't have house N2 gas. What we do is to use the gas outlet from a Taylor-Wharton 250L liquid nitrogen tank, with a higher pressure regulator (set to 6 bar max). It is not a standard usage of the tank, but it works for us. One thing to note is the cold N2 gas outlet needs to be warmed up to prevent crazy condensation. One tank lasts more than 1 week. While it may be hard to measure the water content in the N2 gas outlet, and it may depend on the cleanliness of the liquid N2, but our setup allows continuous usage of the Aquilos2 for multiple days.

Tat



Multiscale Bioimaging Cluster of Excellence
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On 05.07.23 20:54, Sasha Dickinson wrote:
Hi Xinran,

We also do not have house N2 gas so I tried a few different N2 sources and found that the best / most reasonable one was a standard ~5000 cubic feet tank at 350 PSI (as opposed to the typical 22 PSI). This tank usually lasts us >1 week on our Aquilos 2, so I usually have it replaced weekly like the ones for the cryoTEMs.

Very best,
Sasha

On Wed, Jul 5, 2023 at 11:33 AM Liu, Xinran <xinran.liu at yale.edu<mailto:xinran.liu at yale.edu>> wrote:
Dear Colleagues,

I am looking for help to identify a dry N2 generator that can work with the Aquilos II cryo FIB (Thermo Fisher).

It has become a concern that the Aquilos milling operation requires a huge volume of dry nitrogen that makes use of typical cylinders not practical. Because our building does not have a N2 supply source, we will have to purchase and install one that works with the Aquilos. Our multiple attempts to reach out to Thermo Fisher have not generated any response so far. The following are requirements for the generator, if someone here knows one or uses one that meet the criteria, can you share the information? much appreciated.

N2 at connection of the microscope
0.5x105 Pa (7.25 psi, 0.5 bar) to 0.8 x105 Pa (11.6 psi, 0.8 bar)
Flow rate is 30 liters/min
The water content must be less than 10 ppm

Xinran Nick Liu, M.D. & Ph.D.
Director, Center for Cellular & Molecular Imaging
Electron Microscopy Core Facility
Senior Research Scientist in Cell Biology
Yale University School of Medicine
Office Phone: 203.785.4050
Lab Phone: 203.785.5390
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