[3dem] [EXTERNAL] 3dem Digest, Vol 185, Issue 36
Galkin, Vitold E.
GalkinVE at EVMS.EDU
Wed Jan 25 09:28:14 PST 2023
Hi Ed,
The PELCO easiGlow Glow Discharge Cleaning System gives very similar results to plasma cleaner. I do not see any difference in the quality of ice between the two.
Regards,
Vitold.
________________________________
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Sent: Wednesday, January 25, 2023 12:15:36 PM
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Subject: [EXTERNAL] 3dem Digest, Vol 185, Issue 36
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Today's Topics:
1. plasma cleaner (Egelman, Edward H (ehe2n))
2. Re: plasma cleaner (Christopher Winkler)
3. cryoEM EMBO course Birkbeck Sept 2023 (Giulia Zanetti (Staff))
4. EPU question (Morgan, David Gene)
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Message: 1
Date: Tue, 24 Jan 2023 22:17:30 +0000
From: "Egelman, Edward H (ehe2n)" <egelman at virginia.edu>
To: "3dem at ncmir.ucsd.edu" <3dem at ncmir.ucsd.edu>
Subject: [3dem] plasma cleaner
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We have a Gatan Solarus that is failing, and are thinking about replacements. The newer Solarus II is from 5-10 times more expensive than the simplest plasma cleaners out there. Does anyone have any comparisons to justify this very high price tag?
Thanks,
Ed
Edward Egelman
Harrison Distinguished Professor,
Department of Biochemistry and Molecular Genetics
Professor of Molecular Physiology and Biological Physics
University of Virginia
phone: 434-924-8210
fax: 434-924-5069
egelman at virginia.edu<mailto:egelman at virginia.edu>
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Message: 2
Date: Tue, 24 Jan 2023 17:43:35 -0500
From: Christopher Winkler <microwink at gmail.com>
To: "Egelman, Edward H (ehe2n)" <egelman at virginia.edu>
Cc: "3dem at ncmir.ucsd.edu" <3dem at ncmir.ucsd.edu>
Subject: Re: [3dem] plasma cleaner
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Hey Ed,
We went through a similar cost benefit analysis of plasma cleaners after
our Solarus RF board failed. We went with one of the more basic plasma
cleaners partly due to budget, partly due to the simplicity of operation of
the more basic units, and partly due to the simplicity of design of the
basic units. As you point out, the cost of a "simple" cleaner is 1/4 to 1/3
of the Solarus II. The Solarus was pretty easy to operate and the ability
to save recipes is nice, but the more basic plasma cleaners are even
simpler--you basically toggle a switch to start or stop the cleaner. There
is no ability to save a recipe on our cleaner but we only had a few saved
recipes on our Solarus that were routinely used. It's not hard to adjust
time and power on the basic units. Most importantly for me, the more basic
cleaners forego the TMP for a basic dry scroll pump so there are no
diaphragm pumps to rebuild or turbo oil bearings to swap, and there's no
painful teardown of the system to access the vacuum systems. The poorer
vacuum is fully sufficient to generate a good quality plasma and I've not
really seen much of a difference in cleaning performance--the new "simple"
model does a good job of cleaning and removing hydrocarbon contamination
(we don't use it for glow discharging or treating grid surfaces, so I can't
comment on that).
A downside of the basic cleaner is that it only uses one process gas at a
time, so you cannot switch between argon and oxygen mixtures or hydrogen
like the Solarus. This is not a problem for our application but it's
something to consider.
Happy to discuss specifics off of the listserv,
Chris
On Tue, Jan 24, 2023 at 5:17 PM Egelman, Edward H (ehe2n) <
egelman at virginia.edu> wrote:
> We have a Gatan Solarus that is failing, and are thinking about
> replacements. The newer Solarus II is from 5-10 times more expensive than
> the simplest plasma cleaners out there. Does anyone have any comparisons to
> justify this very high price tag?
>
> Thanks,
>
> Ed
>
>
>
>
>
>
>
> Edward Egelman
>
> Harrison Distinguished Professor,
>
> Department of Biochemistry and Molecular Genetics
>
> Professor of Molecular Physiology and Biological Physics
>
> University of Virginia
>
> phone: 434-924-8210
>
> fax: 434-924-5069
>
> egelman at virginia.edu
>
> https://urldefense.com/v3/__https://egelmanlab.org/__;!!Mih3wA!EQBiSrdldpeZKdG9q0YNedYLR8usKTHGLIJxVHDs09kYZTgnVOnBPj86OJuAm4qW3tF3Vkde-sEGVLuSMC-9$
> <https://urldefense.com/v3/__https://egelmanlab.org/__;!!Mih3wA!E1FbZMjPylp2E_W95tkmjXBa_SrdglqlKHumjIYttQDrp5j2mFygiYldlXjLxNN29KbT4EBRw_E2__08r2FUsiA$>
>
>
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Message: 3
Date: Wed, 25 Jan 2023 08:16:54 +0000
From: "Giulia Zanetti (Staff)" <g.zanetti at bbk.ac.uk>
To: "3dem at ncmir.ucsd.edu" <3dem at ncmir.ucsd.edu>
Subject: [3dem] cryoEM EMBO course Birkbeck Sept 2023
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Dear EM community,
Registrations are now open for the 2023 Birkbeck EMBO practical course on cryoEM and image processing.
This course will cover image processing for single particle and tomography/subtomogram averaging, from basic theory to practical sessions, and will be taught by an outstanding lineup of speakers and demonstrators.
Please apply from the EMBO webpage, deadline 15th May.
https://urldefense.com/v3/__https://meetings.embo.org/event/23-cryo-em-image-processing__;!!Mih3wA!CZnPIpuuRylRichQeP-R4tuWfHGt6ft7TLiwry1XnyJ9bvaCURe5GbeWFwVsfwgPI7bWGBmTRz3jk_V9NDn7smJC$
Applicants will be chosen based on:
* Motivation
* Level of expertise (some familiarity with structural EM and image processing/linux)
* Commitment to cryoEM in the long term
* Supervisor recommendation
We will select a maximum of 1 applicant per institution, and ensure a balanced geographical and gender distribution.
We require a motivation cover letter, a CV and a reference from current supervisor, and an abstract describing current research.
Best wishes
Giulia (on behalf of all organisers)
3dem
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Message: 4
Date: Wed, 25 Jan 2023 17:15:07 +0000
From: "Morgan, David Gene" <dagmorga at indiana.edu>
To: "3dem at ncmir.ucsd.edu" <3dem at ncmir.ucsd.edu>
Subject: [3dem] EPU question
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Hi,
If I select a gridsquare and then maybe a dozen holes using EPU, and acquire those images, is there any way to select more hole from that same grid square without acquiring a new gridsquare image? On our Talos Arctica, I appear to be locked out of any further manipulation of the existing gridsquare image.
Thanks for any advice.
--
politics is more difficult than physics.
A. Einstein
David Gene Morgan
Electron Microscopy Center
047E Simon Hall
IU Bloomington
812 856 1457 (office)
812 856 3221 (3200)
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