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<p>Hi Ed,</p>
<p>The PELCO easiGlow Glow Discharge Cleaning System gives very similar results to plasma cleaner. I do not see any difference in the quality of ice between the two.<br>
</p>
<p>Regards,</p>
<p>Vitold.</p>
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<div id="x_divRplyFwdMsg" dir="ltr"><font face="Calibri, sans-serif" color="#000000" style="font-size:11pt"><b>From:</b> 3dem <3dem-bounces@ncmir.ucsd.edu> on behalf of 3dem-request@ncmir.ucsd.edu <3dem-request@ncmir.ucsd.edu><br>
<b>Sent:</b> Wednesday, January 25, 2023 12:15:36 PM<br>
<b>To:</b> 3dem@ncmir.ucsd.edu<br>
<b>Subject:</b> [EXTERNAL] 3dem Digest, Vol 185, Issue 36</font>
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Today's Topics:<br>
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1. plasma cleaner (Egelman, Edward H (ehe2n))<br>
2. Re: plasma cleaner (Christopher Winkler)<br>
3. cryoEM EMBO course Birkbeck Sept 2023 (Giulia Zanetti (Staff))<br>
4. EPU question (Morgan, David Gene)<br>
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----------------------------------------------------------------------<br>
<br>
Message: 1<br>
Date: Tue, 24 Jan 2023 22:17:30 +0000<br>
From: "Egelman, Edward H (ehe2n)" <egelman@virginia.edu><br>
To: "3dem@ncmir.ucsd.edu" <3dem@ncmir.ucsd.edu><br>
Subject: [3dem] plasma cleaner<br>
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We have a Gatan Solarus that is failing, and are thinking about replacements. The newer Solarus II is from 5-10 times more expensive than the simplest plasma cleaners out there. Does anyone have any comparisons to justify this very high price tag?<br>
Thanks,<br>
Ed<br>
<br>
<br>
<br>
Edward Egelman<br>
Harrison Distinguished Professor,<br>
Department of Biochemistry and Molecular Genetics<br>
Professor of Molecular Physiology and Biological Physics<br>
University of Virginia<br>
phone: 434-924-8210<br>
fax: 434-924-5069<br>
egelman@virginia.edu<mailto:egelman@virginia.edu><br>
<a href="https://urldefense.com/v3/__https://egelmanlab.org/__;!!Mih3wA!E1FbZMjPylp2E_W95tkmjXBa_SrdglqlKHumjIYttQDrp5j2mFygiYldlXjLxNN29KbT4EBRw_E2__08r2FUsiA$">https://urldefense.com/v3/__https://egelmanlab.org/__;!!Mih3wA!E1FbZMjPylp2E_W95tkmjXBa_SrdglqlKHumjIYttQDrp5j2mFygiYldlXjLxNN29KbT4EBRw_E2__08r2FUsiA$</a>
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Message: 2<br>
Date: Tue, 24 Jan 2023 17:43:35 -0500<br>
From: Christopher Winkler <microwink@gmail.com><br>
To: "Egelman, Edward H (ehe2n)" <egelman@virginia.edu><br>
Cc: "3dem@ncmir.ucsd.edu" <3dem@ncmir.ucsd.edu><br>
Subject: Re: [3dem] plasma cleaner<br>
Message-ID:<br>
<CAA8T2PO4biKfy4rFcBHknj=hJKopQh9OU5bnnZE894Qu04sPTA@mail.gmail.com><br>
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<br>
Hey Ed,<br>
<br>
We went through a similar cost benefit analysis of plasma cleaners after<br>
our Solarus RF board failed. We went with one of the more basic plasma<br>
cleaners partly due to budget, partly due to the simplicity of operation of<br>
the more basic units, and partly due to the simplicity of design of the<br>
basic units. As you point out, the cost of a "simple" cleaner is 1/4 to 1/3<br>
of the Solarus II. The Solarus was pretty easy to operate and the ability<br>
to save recipes is nice, but the more basic plasma cleaners are even<br>
simpler--you basically toggle a switch to start or stop the cleaner. There<br>
is no ability to save a recipe on our cleaner but we only had a few saved<br>
recipes on our Solarus that were routinely used. It's not hard to adjust<br>
time and power on the basic units. Most importantly for me, the more basic<br>
cleaners forego the TMP for a basic dry scroll pump so there are no<br>
diaphragm pumps to rebuild or turbo oil bearings to swap, and there's no<br>
painful teardown of the system to access the vacuum systems. The poorer<br>
vacuum is fully sufficient to generate a good quality plasma and I've not<br>
really seen much of a difference in cleaning performance--the new "simple"<br>
model does a good job of cleaning and removing hydrocarbon contamination<br>
(we don't use it for glow discharging or treating grid surfaces, so I can't<br>
comment on that).<br>
<br>
A downside of the basic cleaner is that it only uses one process gas at a<br>
time, so you cannot switch between argon and oxygen mixtures or hydrogen<br>
like the Solarus. This is not a problem for our application but it's<br>
something to consider.<br>
<br>
Happy to discuss specifics off of the listserv,<br>
Chris<br>
<br>
On Tue, Jan 24, 2023 at 5:17 PM Egelman, Edward H (ehe2n) <<br>
egelman@virginia.edu> wrote:<br>
<br>
> We have a Gatan Solarus that is failing, and are thinking about<br>
> replacements. The newer Solarus II is from 5-10 times more expensive than<br>
> the simplest plasma cleaners out there. Does anyone have any comparisons to<br>
> justify this very high price tag?<br>
><br>
> Thanks,<br>
><br>
> Ed<br>
><br>
><br>
><br>
><br>
><br>
><br>
><br>
> Edward Egelman<br>
><br>
> Harrison Distinguished Professor,<br>
><br>
> Department of Biochemistry and Molecular Genetics<br>
><br>
> Professor of Molecular Physiology and Biological Physics<br>
><br>
> University of Virginia<br>
><br>
> phone: 434-924-8210<br>
><br>
> fax: 434-924-5069<br>
><br>
> egelman@virginia.edu<br>
><br>
> <a href="https://urldefense.com/v3/__https://egelmanlab.org/__;!!Mih3wA!EQBiSrdldpeZKdG9q0YNedYLR8usKTHGLIJxVHDs09kYZTgnVOnBPj86OJuAm4qW3tF3Vkde-sEGVLuSMC-9$">
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------------------------------<br>
<br>
Message: 3<br>
Date: Wed, 25 Jan 2023 08:16:54 +0000<br>
From: "Giulia Zanetti (Staff)" <g.zanetti@bbk.ac.uk><br>
To: "3dem@ncmir.ucsd.edu" <3dem@ncmir.ucsd.edu><br>
Subject: [3dem] cryoEM EMBO course Birkbeck Sept 2023<br>
Message-ID:<br>
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<br>
Dear EM community,<br>
<br>
Registrations are now open for the 2023 Birkbeck EMBO practical course on cryoEM and image processing.<br>
This course will cover image processing for single particle and tomography/subtomogram averaging, from basic theory to practical sessions, and will be taught by an outstanding lineup of speakers and demonstrators.<br>
<br>
Please apply from the EMBO webpage, deadline 15th May.<br>
<a href="https://urldefense.com/v3/__https://meetings.embo.org/event/23-cryo-em-image-processing__;!!Mih3wA!CZnPIpuuRylRichQeP-R4tuWfHGt6ft7TLiwry1XnyJ9bvaCURe5GbeWFwVsfwgPI7bWGBmTRz3jk_V9NDn7smJC$">https://urldefense.com/v3/__https://meetings.embo.org/event/23-cryo-em-image-processing__;!!Mih3wA!CZnPIpuuRylRichQeP-R4tuWfHGt6ft7TLiwry1XnyJ9bvaCURe5GbeWFwVsfwgPI7bWGBmTRz3jk_V9NDn7smJC$</a>
<br>
<br>
Applicants will be chosen based on:<br>
<br>
* Motivation<br>
* Level of expertise (some familiarity with structural EM and image processing/linux)<br>
* Commitment to cryoEM in the long term<br>
* Supervisor recommendation<br>
We will select a maximum of 1 applicant per institution, and ensure a balanced geographical and gender distribution.<br>
We require a motivation cover letter, a CV and a reference from current supervisor, and an abstract describing current research.<br>
<br>
Best wishes<br>
<br>
Giulia (on behalf of all organisers)<br>
<br>
3dem<br>
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<br>
Message: 4<br>
Date: Wed, 25 Jan 2023 17:15:07 +0000<br>
From: "Morgan, David Gene" <dagmorga@indiana.edu><br>
To: "3dem@ncmir.ucsd.edu" <3dem@ncmir.ucsd.edu><br>
Subject: [3dem] EPU question<br>
Message-ID:<br>
<BN8PR08MB640448E71524ABB9A2CB4C7FD0CE9@BN8PR08MB6404.namprd08.prod.outlook.com><br>
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Content-Type: text/plain; charset="iso-8859-1"<br>
<br>
Hi,<br>
<br>
If I select a gridsquare and then maybe a dozen holes using EPU, and acquire those images, is there any way to select more hole from that same grid square without acquiring a new gridsquare image? On our Talos Arctica, I appear to be locked out of any further
manipulation of the existing gridsquare image.<br>
<br>
Thanks for any advice.<br>
<br>
--<br>
politics is more difficult than physics.<br>
A. Einstein<br>
<br>
David Gene Morgan<br>
Electron Microscopy Center<br>
047E Simon Hall<br>
IU Bloomington<br>
812 856 1457 (office)<br>
812 856 3221 (3200)<br>
<a href="https://urldefense.com/v3/__http://iubemcenter.indiana.edu__;!!Mih3wA!BIHJTFu4hIRPUFD_-ESAWx-P3SRMmAs23_epCApFGtpP8acvv_O1T0_5uE9pSiCWiiQ6EVDJsKdoOrfuQgGAUTM$">
https://urldefense.com/v3/__http://iubemcenter.indiana.edu__;!!Mih3wA!BIHJTFu4hIRPUFD_-ESAWx-P3SRMmAs23_epCApFGtpP8acvv_O1T0_5uE9pSiCWiiQ6EVDJsKdoOrfuQgGAUTM$</a>
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