[3dem] PhD position - Horizon doctoral network - for "In situ imaging of intact Cochlea stereocilia by correlative 3D cryo-microscopies techniques" at Institut Pasteur, Paris

[Anna Sartori Rupp]

Dear all,



We have *one PhD position* available between the lab of Nicolas Wolff
<https://urldefense.com/v3/__https://research.pasteur.fr/fr/member/nicolas-wolff/__;!!Mih3wA!A_1rAwxnGexoxRC-BpxNB4ccu8KdVa6bygCK6xE140VhPIkZvR8yeiopxQmnmHt_b3KjFnq0RN68PYwjXHkoEjogsrxZgQ$ > - Signalling and
Molecular Interactions group, and the one of Anna Sartori-Rupp
<https://urldefense.com/v3/__https://research.pasteur.fr/en/member/anna-sartori-rupp/__;!!Mih3wA!A_1rAwxnGexoxRC-BpxNB4ccu8KdVa6bygCK6xE140VhPIkZvR8yeiopxQmnmHt_b3KjFnq0RN68PYwjXHkoEjqOR-k8xQ$ > at the NanoImaging
cryo-EM facility <https://urldefense.com/v3/__https://research.pasteur.fr/en/team/nanoimaging/__;!!Mih3wA!A_1rAwxnGexoxRC-BpxNB4ccu8KdVa6bygCK6xE140VhPIkZvR8yeiopxQmnmHt_b3KjFnq0RN68PYwjXHkoEjoDBGf0Rg$ > of Institut
Pasteur <https://urldefense.com/v3/__https://www.pasteur.fr/fr__;!!Mih3wA!A_1rAwxnGexoxRC-BpxNB4ccu8KdVa6bygCK6xE140VhPIkZvR8yeiopxQmnmHt_b3KjFnq0RN68PYwjXHkoEjrzZxuUEA$ > in Paris for *“In situ imaging of the
protein complex Usher 2 involved in hearing and vision in intact Cochlea
stereocilia”* by using *correlative 3D microscopy techniques in cryogenic
conditions* (cryo-super-resolution light microscopy, cryo-electron
tomography and cryo-soft X-ray tomography) on native tissue samples.

The project aims to address the general structure in situ of the native
protein complex Usher 2, needed for the development of the cochlea, the
organ of hearing, and to elucidate the impact of deafness mutations in
pathological processes. The Usher 2 syndrome is the most common form of
hereditary hearing-vision loss in humans. We propose to combine cutting
edge imaging approaches using cryo-electron tomography (*cryoET*),
cryo-soft-X-ray tomography (*cryoSXT*), cryo-correlative light/X-rays and
electron microscopy (*cryoCLEXM*) and super-resolution fluorescence
microscopy (*STED*) on mouse cochlea samples to dissect the 3D organization
of the Usher 2 complex in its native tissue environment. Combined with the
atomic resolution structures already obtained in the lab by integrative
approaches (Cryo-EM, NMR and X-ray diffraction), we will then be able to
provide a comprehensive view of the Usher 2 complex in situ. Altogether,
our structural results from the micro-microscale (cochlea) to the nano- and
atomic scale (protein domains) will lead us to propose a model on how Usher
2 proteins and partners get organised in situ to form a protein network
essential for the correct Cochlea development. This molecular
characterization will have a potential impact on Usher 2 syndrome diagnosis
and provide a deeper understanding of its physiopathology.

The project is fully funded by Horizon Europe under the Marie
Skłodowska-Curie Actions Doctoral Network (MSCA-DN) programme *CLEXM*
<https://urldefense.com/v3/__https://clexm.eu/__;!!Mih3wA!A_1rAwxnGexoxRC-BpxNB4ccu8KdVa6bygCK6xE140VhPIkZvR8yeiopxQmnmHt_b3KjFnq0RN68PYwjXHkoEjpKz4GEHQ$ >. We have one out of nine PhD positions to offer in the
field of correlative cryo-microscopy *(Project 9, https://urldefense.com/v3/__https://clexm.eu/__;!!Mih3wA!A_1rAwxnGexoxRC-BpxNB4ccu8KdVa6bygCK6xE140VhPIkZvR8yeiopxQmnmHt_b3KjFnq0RN68PYwjXHkoEjpKz4GEHQ$ 
<https://urldefense.com/v3/__https://clexm.eu/__;!!Mih3wA!A_1rAwxnGexoxRC-BpxNB4ccu8KdVa6bygCK6xE140VhPIkZvR8yeiopxQmnmHt_b3KjFnq0RN68PYwjXHkoEjpKz4GEHQ$ >)*. It will be carried out in close collaboration with
the partners in Barcelona, Heidelberg, Dublin and Aalen with the aim of
developing multimodal correlative imaging pipelines to ultimately help
understanding disease and develop effective therapies.

Apply and join us on this exciting project in a unique scientific
environment in the very heart of Paris!

*Application deadline for our project is January 31st, 2024.*

*To apply*, *please directly send your CV*, including personal contact
details, *and a cover letter* including the contact details of 2 referees
*to*:

nicolas.wolff at pasteur.fr

anna.sartori-rupp at pasteur.fr

Do not hesitate to contact us at the email addresses listed above if you
have any questions!

Please help spreading the word,

Cheers

Anna & Nicolas

For more details about the labs, please visit:

https://urldefense.com/v3/__https://research.pasteur.fr/fr/member/nicolas-wolff/__;!!Mih3wA!A_1rAwxnGexoxRC-BpxNB4ccu8KdVa6bygCK6xE140VhPIkZvR8yeiopxQmnmHt_b3KjFnq0RN68PYwjXHkoEjogsrxZgQ$ 

https://urldefense.com/v3/__https://research.pasteur.fr/en/member/anna-sartori-rupp/__;!!Mih3wA!A_1rAwxnGexoxRC-BpxNB4ccu8KdVa6bygCK6xE140VhPIkZvR8yeiopxQmnmHt_b3KjFnq0RN68PYwjXHkoEjqOR-k8xQ$ 

https://urldefense.com/v3/__https://research.pasteur.fr/en/team/nanoimaging/__;!!Mih3wA!A_1rAwxnGexoxRC-BpxNB4ccu8KdVa6bygCK6xE140VhPIkZvR8yeiopxQmnmHt_b3KjFnq0RN68PYwjXHkoEjoDBGf0Rg$ 
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