<div dir="ltr"><div class="gmail_default" style="font-family:arial,helvetica,sans-serif"><p style="margin:0cm;line-height:115%;background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-origin:initial;background-clip:initial;font-size:11pt;font-family:Calibri,sans-serif"><a name="m_-7340645144118659933__Hlk153357570"><span lang="EN-GB" style="color:black;background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-origin:initial;background-clip:initial">Dear
all,</span></a></p>

<p style="margin:0cm;line-height:115%;background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-origin:initial;background-clip:initial;font-size:11pt;font-family:Calibri,sans-serif"><span lang="EN-GB" style="color:black;background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-origin:initial;background-clip:initial"> </span></p>

<p style="margin:0cm;line-height:115%;background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-origin:initial;background-clip:initial;font-size:11pt;font-family:Calibri,sans-serif"><span lang="EN-GB" style="color:black;background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-origin:initial;background-clip:initial">We have <b>one PhD position</b> available between the lab of </span><a href="https://urldefense.com/v3/__https://research.pasteur.fr/fr/member/nicolas-wolff/__;!!Mih3wA!A_1rAwxnGexoxRC-BpxNB4ccu8KdVa6bygCK6xE140VhPIkZvR8yeiopxQmnmHt_b3KjFnq0RN68PYwjXHkoEjogsrxZgQ$" style="color:rgb(5,99,193)" target="_blank"><span lang="EN-GB" style="background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-origin:initial;background-clip:initial">Nicolas Wolff</span></a><span lang="EN-GB" style="color:black;background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-origin:initial;background-clip:initial"> - Signalling
and Molecular Interactions group, and the one of </span><a href="https://urldefense.com/v3/__https://research.pasteur.fr/en/member/anna-sartori-rupp/__;!!Mih3wA!A_1rAwxnGexoxRC-BpxNB4ccu8KdVa6bygCK6xE140VhPIkZvR8yeiopxQmnmHt_b3KjFnq0RN68PYwjXHkoEjqOR-k8xQ$" style="color:rgb(5,99,193)" target="_blank"><span lang="EN-GB">Anna </span><span><span lang="EN-GB" style="text-decoration-line:none">Sartori</span></span><span lang="EN-GB">-</span><span><span lang="EN-GB" style="text-decoration-line:none">Rupp</span></span></a><span lang="EN-GB"> at the </span><a href="https://urldefense.com/v3/__https://research.pasteur.fr/en/team/nanoimaging/__;!!Mih3wA!A_1rAwxnGexoxRC-BpxNB4ccu8KdVa6bygCK6xE140VhPIkZvR8yeiopxQmnmHt_b3KjFnq0RN68PYwjXHkoEjoDBGf0Rg$" style="color:rgb(5,99,193)" target="_blank"><span lang="EN-GB">NanoImaging cryo-EM facility</span></a><span lang="EN-GB"> of </span><a href="https://urldefense.com/v3/__https://www.pasteur.fr/fr__;!!Mih3wA!A_1rAwxnGexoxRC-BpxNB4ccu8KdVa6bygCK6xE140VhPIkZvR8yeiopxQmnmHt_b3KjFnq0RN68PYwjXHkoEjrzZxuUEA$" style="color:rgb(5,99,193)" target="_blank"><span lang="EN-GB">Institut Pasteur</span></a><span lang="EN-GB"> in Paris</span><span lang="EN-GB" style="color:black;background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-origin:initial;background-clip:initial"> for <b><i>“In situ imaging of the
protein complex Usher 2 involved in hearing and vision in intact Cochlea
stereocilia”</i></b> by using <i><u>correlative</u> <u>3D microscopy techniques
in cryogenic conditions</u></i><u> </u>(cryo-super-resolution light microscopy,
cryo-electron tomography and cryo-soft X-ray tomography) on native tissue
samples.</span></p>

<p style="line-height:115%;background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-origin:initial;background-clip:initial;margin-right:0cm;margin-left:0cm;font-size:11pt;font-family:Calibri,sans-serif"><span lang="EN-GB" style="color:black">The
project aims to address the general structure in situ of the native protein
complex Usher 2, needed for the development of the cochlea, the organ of
hearing, and to elucidate the impact of deafness mutations in pathological
processes. The Usher 2 syndrome is the most common form of hereditary
hearing-vision loss in humans. We propose to combine cutting edge imaging
approaches using cryo-electron tomography (<b>cryoET</b>), cryo-soft-X-ray
tomography (<b>cryoSXT</b>), cryo-correlative light/X-rays and electron
microscopy (<b>cryoCLEXM</b>) and super-resolution fluorescence microscopy (<b>STED</b>)
on mouse cochlea samples to dissect the 3D organization of the Usher 2 complex
in its native tissue environment. Combined with the atomic resolution
structures already obtained in the lab by integrative approaches (Cryo-EM, NMR
and X-ray diffraction), we will then be able to provide a comprehensive view of
the Usher 2 complex in situ. Altogether, our structural results from the
micro-microscale (cochlea) to the nano- and atomic scale (protein domains) will
lead us to propose a model on how Usher 2 proteins and partners get organised
in situ to form a protein network essential for the correct Cochlea
development. This molecular characterization will have a potential impact on
Usher 2 syndrome diagnosis and provide a deeper understanding of its
physiopathology.<br>
</span><span lang="EN-GB" style="font-size:10.5pt;line-height:115%;font-family:"Segoe UI",sans-serif;color:black"><br>
</span><span lang="EN-GB" style="color:black">The project is fully funded by
Horizon Europe under the Marie Skłodowska-Curie Actions Doctoral Network
(MSCA-DN) programme </span><a href="https://urldefense.com/v3/__https://clexm.eu/__;!!Mih3wA!A_1rAwxnGexoxRC-BpxNB4ccu8KdVa6bygCK6xE140VhPIkZvR8yeiopxQmnmHt_b3KjFnq0RN68PYwjXHkoEjpKz4GEHQ$" style="color:rgb(5,99,193)" target="_blank"><b><span lang="EN-GB">CLEXM</span></b></a><span lang="EN-GB" style="color:black">. We have one out of nine PhD positions to offer in
the field of correlative cryo-microscopy <b>(Project 9, <a href="https://urldefense.com/v3/__https://clexm.eu/__;!!Mih3wA!A_1rAwxnGexoxRC-BpxNB4ccu8KdVa6bygCK6xE140VhPIkZvR8yeiopxQmnmHt_b3KjFnq0RN68PYwjXHkoEjpKz4GEHQ$" target="_blank">https://clexm.eu/</a>)</b>.
It will be carried out in close collaboration with the partners in Barcelona, Heidelberg,
Dublin and Aalen with the aim of developing multimodal correlative imaging
pipelines to ultimately help understanding disease and develop effective
therapies. </span></p>

<p class="MsoNormal" style="margin:0cm 0cm 8pt;background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-origin:initial;background-clip:initial;font-size:11pt;font-family:Calibri,sans-serif"><span lang="EN-GB">Apply and join us on this exciting project in a unique
scientific environment in the very heart of Paris!</span></p>

<p style="line-height:115%;background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-origin:initial;background-clip:initial;margin-right:0cm;margin-left:0cm;font-size:11pt;font-family:Calibri,sans-serif"><b><span lang="EN-GB" style="color:black">Application deadline for
our project is January 31^st, 2024.</span></b></p>

<p style="line-height:115%;background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-origin:initial;background-clip:initial;margin-right:0cm;margin-left:0cm;font-size:11pt;font-family:Calibri,sans-serif"><b><span lang="EN-GB" style="color:black">To apply</span></b><span lang="EN-GB" style="color:black">, <b>please directly send your CV</b>, including
personal contact details, <b>and a cover letter</b> including the contact
details of 2 referees <b>to</b>:</span></p>

<p style="line-height:115%;background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-origin:initial;background-clip:initial;margin-right:0cm;margin-left:0cm;font-size:11pt;font-family:Calibri,sans-serif"><a href="mailto:nicolas.wolff@pasteur.fr" style="color:rgb(5,99,193)" target="_blank"><span lang="EN-GB">nicolas.wolff@pasteur.fr</span></a><span lang="EN-GB" style="color:black"></span></p>

<p style="line-height:115%;background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-origin:initial;background-clip:initial;margin-right:0cm;margin-left:0cm;font-size:11pt;font-family:Calibri,sans-serif"><a href="mailto:anna.sartori-rupp@pasteur.fr" style="color:rgb(5,99,193)" target="_blank"><span lang="EN-GB">anna.sartori-rupp@pasteur.fr</span></a><span lang="EN-GB" style="color:black"></span></p>

<p style="line-height:115%;background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-origin:initial;background-clip:initial;margin-right:0cm;margin-left:0cm;font-size:11pt;font-family:Calibri,sans-serif"><span lang="EN-GB" style="color:black">Do not hesitate to
contact us at the email addresses listed above if you have any questions!  </span></p>

<p style="line-height:115%;background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-origin:initial;background-clip:initial;margin-right:0cm;margin-left:0cm;font-size:11pt;font-family:Calibri,sans-serif"><span lang="EN-GB" style="color:black;background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-origin:initial;background-clip:initial">Please
help spreading the word,</span><span lang="EN-GB" style="color:black"></span></p>

<p style="line-height:115%;background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-origin:initial;background-clip:initial;margin-right:0cm;margin-left:0cm;font-size:11pt;font-family:Calibri,sans-serif"><span lang="EN-GB" style="color:black">Cheers</span></p>

<p style="line-height:115%;background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-origin:initial;background-clip:initial;margin-right:0cm;margin-left:0cm;font-size:11pt;font-family:Calibri,sans-serif"><span lang="EN-GB" style="color:black">Anna & Nicolas</span></p>

<br>

<p class="MsoNormal" style="background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-origin:initial;background-clip:initial;margin:0cm;font-size:11pt;font-family:Calibri,sans-serif"><span lang="EN-GB">For more details about
the labs, please visit:</span><span lang="EN-GB"></span></p>

<p style="background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-origin:initial;background-clip:initial;margin-right:0cm;margin-left:0cm;font-size:11pt;font-family:Calibri,sans-serif"><a href="https://urldefense.com/v3/__https://research.pasteur.fr/fr/member/nicolas-wolff/__;!!Mih3wA!A_1rAwxnGexoxRC-BpxNB4ccu8KdVa6bygCK6xE140VhPIkZvR8yeiopxQmnmHt_b3KjFnq0RN68PYwjXHkoEjogsrxZgQ$" style="color:rgb(5,99,193)" target="_blank"><span lang="EN-GB">https://research.pasteur.fr/fr/member/nicolas-wolff/</span></a><span lang="EN-GB"></span></p>

<p style="background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-origin:initial;background-clip:initial;margin-right:0cm;margin-left:0cm;font-size:11pt;font-family:Calibri,sans-serif"><a href="https://urldefense.com/v3/__https://research.pasteur.fr/en/member/anna-sartori-rupp/__;!!Mih3wA!A_1rAwxnGexoxRC-BpxNB4ccu8KdVa6bygCK6xE140VhPIkZvR8yeiopxQmnmHt_b3KjFnq0RN68PYwjXHkoEjqOR-k8xQ$" style="color:rgb(5,99,193)" target="_blank"><span lang="EN-GB">https://research.pasteur.fr/en/member/anna-sartori-rupp/</span></a><span lang="EN-GB"></span></p>

<p style="background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-origin:initial;background-clip:initial;margin-right:0cm;margin-left:0cm;font-size:11pt;font-family:Calibri,sans-serif"><a href="https://urldefense.com/v3/__https://research.pasteur.fr/en/team/nanoimaging/__;!!Mih3wA!A_1rAwxnGexoxRC-BpxNB4ccu8KdVa6bygCK6xE140VhPIkZvR8yeiopxQmnmHt_b3KjFnq0RN68PYwjXHkoEjoDBGf0Rg$" style="color:rgb(5,99,193)" target="_blank"><span lang="EN-GB">https://research.pasteur.fr/en/team/nanoimaging/</span></a><span lang="EN-GB"></span></p></div></div>