[3dem] Vibrations with cryo-holders

[Michael Elbaum]

Hi Denis,   I had meant to write earlier. We also had vibration problems that were amplified by having a large monitor sitting on the microscope table. It acted like an acoustic antenna. Mounting the monitor from behind without contact to the table did wonders.
Best wishes,
Michael
________________________________
From: 3dem <3dem-bounces at ncmir.ucsd.edu> on behalf of Denis Chrétien <denis.chretien at univ-rennes.fr>
Sent: Thursday, August 24, 2023 8:14:21 PM
To: 3dem at ncmir.ucsd.edu <3dem at ncmir.ucsd.edu>
Subject: Re: [3dem] Vibrations with cryo-holders

Dear all,

Just a quick update since we solved the problem, a big thank to Matthias Stumpf from Simple Origin for his help.

The answer was a sound coming from the fans of the Power Supply of the microscope at a frequency of 393 Hz, see image below (the dotted line in the temporal FFT). This was recorded on my cell phone (Android) with the Spectroid app (unsure it is available for iPhones …).

Hope this can be helpful for the few dinosaurs (as me) who still use side-entry cryo-electron microscopes ;-)

Best wishes,

Denis


 [Frequency.jpeg]
[Power_Supply.jpg]
___________________________
Université de Rennes 1
UMR 6290, équipe MiToS
Campus de Beaulieu, Bât. 13
35042 Rennes Cedex
France

Tel. +33 (0)2 23 23 67 64
https://urldefense.com/v3/__https://igdr.univ-rennes.fr/__;!!Mih3wA!Eqp7Jgypx97OxBCG_SvhhCjaMg4i6zhgJl8Z_PxS1FQVCxfhAVVSkat3s4E_gAs_hva82J1Xb80evYm9pjqeRE91JVJrdn2K3A$ <https://urldefense.com/v3/__https://igdr.univ-rennes.fr/__;!!Mih3wA!CEFkgn0hJSycjadixYwP-zxha6GTnSHugTUDlsDKP8csCg3G8IqZJwg7CE--eQ8BzJHgE5tdP6_FiLX-ONcAyMJ0JagLHHFiEA$>
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Le 9 août 2023 à 16:34, Denis Chrétien <denis.chretien at univ-rennes.fr> a écrit :

Dear cryo-EM community,

We face a recent problem with our cryo-holders, since we get « vibrations" independently of wether they are filled with LN2 or not (see attached movie, look at the FFT). This happens with our 3 cryo-holders (Gatan 626DH, CT3500TR, Simple Origin) but not with the room-temperature holder …

I’ve tried to tackle different sources of noise ans vibrations, but without success. Stoping with air conditioning doesn’t help. I’m wondering wether this is not related with the stage of the microscope itself (Tecnai Sphera 200 kV).

Has someone faced a similar issue and found a solution? Any advice would be warmly appreciated ;-)

Best,

Denis

<Vibrations_cryo.mp4>


___________________________
Université de Rennes 1
UMR 6290, équipe MiToS
Campus de Beaulieu, Bât. 13
35042 Rennes Cedex
France

Tel. +33 (0)2 23 23 67 64
https://urldefense.com/v3/__https://igdr.univ-rennes.fr/__;!!Mih3wA!Eqp7Jgypx97OxBCG_SvhhCjaMg4i6zhgJl8Z_PxS1FQVCxfhAVVSkat3s4E_gAs_hva82J1Xb80evYm9pjqeRE91JVJrdn2K3A$ <https://urldefense.com/v3/__https://igdr.univ-rennes.fr/__;!!Mih3wA!CEFkgn0hJSycjadixYwP-zxha6GTnSHugTUDlsDKP8csCg3G8IqZJwg7CE--eQ8BzJHgE5tdP6_FiLX-ONcAyMJ0JagLHHFiEA$>
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