[3dem] acetone-reistent fluorescence beads for cryo?

[Tobias Furstenhaupt]

Dear colleagues,

for a project we are currently dipping our toes into cryo-CLEM and want to image cells before AFS and Epon embedding by cryo LM to confirm a certain fluorescent protein to be expressed and target this cell for ultramicrotomy. For that we would like to use fluorescent markers/beads next to the cells to serve as fiducials for us to find back our cells of interest after resin embedding. Do you have by any chance an idea of fluorescent markers that emit fluorescence under cryo-conditions and are also acetone resistant to survive the freeze-substitution? QDots maybe? Any successful or failed experiment would be highly valued 

thanks alot in advance :)
Tobias


----------------------------------- 
Tobias Fürstenhaupt, PhD 
head of Electron Microscopy 
Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG) 
Pfotenhauerstrasse 108 
01307 Dresden, Germany 

mail: furstenh at mpi-cbg.de 
phone: (+49) (0)351 210-2690 
cell: (+49) (0)176 / 44498706 (NEW!)