[3dem] Calling all groups who use cryogen mix from Linde-Gas (Germany, maybe Austria, Switzerland?) for plunging
Dario Saczko-Brack
dariobrack at googlemail.com
Tue Apr 25 07:11:09 PDT 2023
Hi Oliver/Cornelia,
if you fill ethan and/or propan in their liquid phase at 200bar, a 10L
cylinder is sufficient.
Best
Dario
On 25.04.23 16:05, Oliver Hofnagel wrote:
>
> Hi Cornelia,
>
> the ethane/propane gas mixture cylinders are filled with only 7 bar
> (pure ethane cylinders with 33 bar). Therefore the recommendation to
> order 50 l cylinders !
> Greetings,
>
> Oliver
>
>
> Dr. Oliver Hofnagel
> Max-Planck-Institute of Molecular Physiology
> Electron Microscopy
> Otto-Hahn-Str. 11
> 44227 Dortmund
> Germany
>
>
>
>
> ------------------------------------------------------------------------
> *Von:* Cornelia Cazey <cornelia.cazey at cssb-hamburg.de>
> *Gesendet:* Dienstag, 25. April 2023 13:50:10
> *An:* Oliver Hofnagel
> *Cc:* Michael Elbaum; r.i.koning at lumc.nl; 3dem at ncmir.ucsd.edu
> *Betreff:* Re: [3dem] Calling all groups who use cryogen mix from
> Linde-Gas (Germany, maybe Austria, Switzerland?) for plunging
> Hi Oliver,
>
> Thanks a lot for the info. But why do they only supply in 50l
> cylinders? Or rather why order a 50l cylinder? Economic reasons?
>
> Thanks!
>
> Cornelia
> On 25 Apr 2023, at 13:40, Oliver Hofnagel
> <oliver.hofnagel at mpi-dortmund.mpg.de> wrote:
>
> Dear Cornelia,
>
>
> here our supplier for 37% ethane / 63% propane 50l cylinder
> cryogen mix in Germany:
>
> /
> /
>
> /AIR LIQUIDE Deutschland GmbH
> Kunden-Service-Center Spezialgase
> Bataverstraße 47
> 47809 Krefeld
> Telefon: (02151) 954-254
> //Email : spezialgase.ald at airliquide.com/
>
>
>
> Its important to order a 50l cylinder, not a 10l cylinder !
>
> Greetings,
>
> Oliver
>
>
> Dr. Oliver Hofnagel
> Max-Planck-Institute of Molecular Physiology
> Electron Microscopy
> Otto-Hahn-Str. 11
> 44227 Dortmund
> Germany
>
>
>
> ------------------------------------------------------------------------
> *Von:* 3dem <3dem-bounces at ncmir.ucsd.edu> im Auftrag von Michael
> Elbaum <michael.elbaum at weizmann.ac.il>
> *Gesendet:* Donnerstag, 20. April 2023 16:29:00
> *An:* r.i.koning at lumc.nl; cornelia.cazey at cssb-hamburg.de;
> 3dem at ncmir.ucsd.edu
> *Betreff:* Re: [3dem] Calling all groups who use cryogen mix from
> Linde-Gas (Germany, maybe Austria, Switzerland?) for plunging
>
> Hi. would it be possible to use two pure tanks and a mixing
> regulator? I think such regulators are used for welding.
>
> Michael
>
> ------------------------------------------------------------------------
> *From:* 3dem <3dem-bounces at ncmir.ucsd.edu> on behalf of
> r.i.koning at lumc.nl <r.i.koning at lumc.nl>
> *Sent:* Thursday, April 20, 2023 5:15:10 PM
> *To:* cornelia.cazey at cssb-hamburg.de; 3dem at ncmir.ucsd.edu
> *Subject:* Re: [3dem] Calling all groups who use cryogen mix from
> Linde-Gas (Germany, maybe Austria, Switzerland?) for plunging
>
> Dear Cornelia,
>
> Our most recent order of ethane/propane also did not come through
> from Line because of the same reason. We will have to do with
> ethane only for now but I prefer the mixture for obvious reasons,
> though we do manage with ethane only. We did not look for nor
> found another vendor.
>
> Best regards,
>
> Roman
>
> *Dr. R.I.Koning*
>
> Leiden University Medical Center, Cell and Chemical Biology,
> Electron Microscopy
>
> Postal Zone S1-P, P.O.Box 9600, 2300 RC, Leiden, The Netherlands
>
> *visiting address.*Einthovenweg 20, Building 2, zone S1-P, room
> R-90-21
>
> *tel.*(31) 71 526 9296 *fax.* (+31) 71 526 8270
> *mail.***r.i.koning at lumc.nl <mailto:r.i.koning at lumc.nl>*web.
> *Electron Microscopy Facility
> <https://urldefense.com/v3/__https://ccb.lumc.nl/electron-microscopy-198__;!!Mih3wA!GxgfkasxXThNdN_k1a9iqn0d9uls-PLaHnrrJAIhFXNITn6ehlzCx_U6V-rQFPvgm8pSGK1CJ1Vd55FxuUK3uvc$>
>
> *From:*3dem <3dem-bounces at ncmir.ucsd.edu> *On Behalf Of *Cazey,
> Cornelia
> *Sent:* donderdag 20 april 2023 15:52
> *To:* 3dem at ncmir.ucsd.edu
> *Subject:* [3dem] Calling all groups who use cryogen mix from
> Linde-Gas (Germany, maybe Austria, Switzerland?) for plunging
>
> Dear list participants,
>
> Our lab is in Germany, and this is a very particular question
> relating to a German supplier, so apologies for addressing the
> entire list, but it seemed the easiest way to find groups who may
> be able to help.
>
> We recently learned that Linde-Gas, our supplier for the cryogen
> mix we use in our facility (37% ethane, rest propane) is no longer
> able to supply this mixture due to the overall difficulties in the
> production, age and poor state of their necessary equipment.
>
> I spoke with the supplier yesterday and while they would like to
> continue to offer the mix, they feel there is not enough market to
> make it feasible. I am now looking for info from groups who are
> using a cryogen mix rather than pure ethane for plunging (I am not
> sure, but relatively convinced that the process would be the same
> for any kind of mixture, so I guess the exact percentages won't
> matter too much) and have previously sourced this from Linde-Gas,
> and might be or have already been affected by this decision. If
> you have been affected, did you find a solution, a different
> supplier or any other alternative you are willing to share? Or if
> you didn't yet know, how much would this news affect your ability
> to work, and would you be interested to try and convince Linde-Gas
> to keep going? I understand the production line has already been
> shut down, but could be revived with enough interest, however, it
> may also come with a price increase (what doesn't).
>
> Any help would be greatly appreciated, whether info on
> alternatives or willingness to push for the supply. I am happy to
> collect replies to send to the Linde-Gas representative in bulk
> or, of course, for those who prefer to contact him directly, do so
> themselves and increase the pressure to keep going/restart production.
>
> Thanks a lot for your help!
>
> Best wishes,
>
> Cornelia Cazey
>
> Cornelia Cazey (She/Her)
>
> Cryo-EM Facility
>
> Centre for Structural Systems Biology (CSSB)
> c/o DESY, Building 15
> Notkestr. 85
> 22607 Hamburg
> Germany
>
>
> phone: +49-(0)40-8998-87706
> email:_cornelia.cazey at cssb-hamburg.de_
>
> /"If you don't know it's impossible, it's easier to do"/ -Neil Gaiman
>
> Sometimes I may send emails outside of core working hours. I do
> not expect you to read, or respond outside of your own working hours!
>
>
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