[3dem] Calling all groups who use cryogen mix from Linde-Gas (Germany, maybe Austria, Switzerland?) for plunging

Tue Apr 25 07:05:00 PDT 2023

Hi Cornelia,

the ethane/propane gas mixture cylinders are filled with only 7 bar (pure ethane cylinders with 33 bar). Therefore the recommendation to order 50 l cylinders !
Greetings,

Oliver

Dr. Oliver Hofnagel
Max-Planck-Institute of Molecular Physiology
Electron Microscopy
Otto-Hahn-Str. 11
44227 Dortmund
Germany

________________________________
Von: Cornelia Cazey <cornelia.cazey at cssb-hamburg.de>
Gesendet: Dienstag, 25. April 2023 13:50:10
An: Oliver Hofnagel
Cc: Michael Elbaum; r.i.koning at lumc.nl; 3dem at ncmir.ucsd.edu
Betreff: Re: [3dem] Calling all groups who use cryogen mix from Linde-Gas (Germany, maybe Austria, Switzerland?) for plunging

Hi Oliver,

Thanks a lot for the info. But why do they only supply in 50l cylinders? Or rather why order a 50l cylinder? Economic reasons?

Thanks!

Cornelia
On 25 Apr 2023, at 13:40, Oliver Hofnagel <oliver.hofnagel at mpi-dortmund.mpg.de<mailto:oliver.hofnagel at mpi-dortmund.mpg.de>> wrote:

Dear Cornelia,

here our supplier for  37% ethane / 63% propane 50l cylinder cryogen mix in Germany:

AIR LIQUIDE Deutschland GmbH
Kunden-Service-Center Spezialgase
Bataverstraße 47
47809 Krefeld
Telefon: (02151) 954-254
Email : spezialgase.ald at airliquide.com

Its important to order a 50l cylinder, not a 10l cylinder !

Greetings,

Oliver

Dr. Oliver Hofnagel
Max-Planck-Institute of Molecular Physiology
Electron Microscopy
Otto-Hahn-Str. 11
44227 Dortmund
Germany

________________________________
Von: 3dem <3dem-bounces at ncmir.ucsd.edu> im Auftrag von Michael Elbaum <michael.elbaum at weizmann.ac.il>
Gesendet: Donnerstag, 20. April 2023 16:29:00
An: r.i.koning at lumc.nl; cornelia.cazey at cssb-hamburg.de; 3dem at ncmir.ucsd.edu
Betreff: Re: [3dem] Calling all groups who use cryogen mix from Linde-Gas (Germany, maybe Austria, Switzerland?) for plunging

Hi.  would it be possible to use two pure tanks and a mixing regulator? I think such regulators are used for welding.

Michael

________________________________
From: 3dem <3dem-bounces at ncmir.ucsd.edu> on behalf of r.i.koning at lumc.nl <r.i.koning at lumc.nl>
Sent: Thursday, April 20, 2023 5:15:10 PM
To: cornelia.cazey at cssb-hamburg.de; 3dem at ncmir.ucsd.edu
Subject: Re: [3dem] Calling all groups who use cryogen mix from Linde-Gas (Germany, maybe Austria, Switzerland?) for plunging

Dear Cornelia,

Our most recent order of ethane/propane also did not come through from Line because of the same reason. We will have to do with ethane only for now but I prefer the mixture for obvious reasons, though we do manage with ethane only. We did not look for nor found another vendor.

Best regards,

Roman

Dr. R.I.Koning

Leiden University Medical Center, Cell and Chemical Biology, Electron Microscopy

Postal Zone S1-P, P.O.Box 9600, 2300 RC, Leiden, The Netherlands

visiting address. Einthovenweg 20, Building 2, zone S1-P, room R-90-21

tel. (31) 71 526 9296 fax. (+31) 71 526 8270 mail. r.i.koning at lumc.nl<mailto:r.i.koning at lumc.nl> web. Electron Microscopy Facility<https://urldefense.com/v3/__https://ccb.lumc.nl/electron-microscopy-198__;!!Mih3wA!GxgfkasxXThNdN_k1a9iqn0d9uls-PLaHnrrJAIhFXNITn6ehlzCx_U6V-rQFPvgm8pSGK1CJ1Vd55FxuUK3uvc$>

From: 3dem <3dem-bounces at ncmir.ucsd.edu> On Behalf Of Cazey, Cornelia
Sent: donderdag 20 april 2023 15:52
To: 3dem at ncmir.ucsd.edu
Subject: [3dem] Calling all groups who use cryogen mix from Linde-Gas (Germany, maybe Austria, Switzerland?) for plunging

Dear list participants,

Our lab is in Germany, and this is a very particular question relating to a German supplier, so apologies for addressing the entire list, but it seemed the easiest way to find groups who may be able to help.

We recently learned that Linde-Gas, our supplier for the cryogen mix we use in our facility (37% ethane, rest propane) is no longer able to supply this mixture due to the overall difficulties in the production, age and poor state of their necessary equipment.

I spoke with the supplier yesterday and while they would like to continue to offer the mix, they feel there is not enough market to make it feasible. I am now looking for info from groups who are using a cryogen mix rather than pure ethane for plunging (I am not sure, but relatively convinced that the process would be the same for any kind of mixture, so I guess the exact percentages won't matter too much) and have previously sourced this from Linde-Gas, and might be or have already been affected by this decision. If you have been affected, did you find a solution, a different supplier or any other alternative you are willing to share? Or if you didn't yet know, how much would this news affect your ability to work, and would you be interested to try and convince Linde-Gas to keep going? I understand the production line has already been shut down, but could be revived with enough interest, however, it may also come with a price increase (what doesn't).

Any help would be greatly appreciated, whether info on alternatives or willingness to push for the supply. I am happy to collect replies to send to the Linde-Gas representative in bulk or, of course, for those who prefer to contact him directly, do so themselves and increase the pressure to keep going/restart production.

Thanks a lot for your help!

Best wishes,

Cornelia Cazey

Cornelia Cazey (She/Her)

Cryo-EM Facility

Centre for Structural Systems Biology (CSSB)
c/o DESY, Building 15
Notkestr. 85
22607 Hamburg
Germany

phone: +49-(0)40-8998-87706
email:cornelia.cazey at cssb-hamburg.de

"If you don't know it's impossible, it's easier to do" -Neil Gaiman

Sometimes I may send emails outside of core working hours. I do not expect you to read, or respond outside of your own working hours!
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