[3dem] Calling all groups who use cryogen mix from Linde-Gas (Germany, maybe Austria, Switzerland?) for plunging

[Cornelia Cazey]

Hi Oliver,

Thanks a lot for the info. But why do they only supply in 50l cylinders? Or rather why order a 50l cylinder? Economic reasons? 

Thanks!

Cornelia

On 25 Apr 2023, 13:40, at 13:40, Oliver Hofnagel <oliver.hofnagel at mpi-dortmund.mpg.de> wrote:
>Dear Cornelia,
>
>
>here our supplier for  37% ethane / 63% propane 50l cylinder cryogen
>mix in Germany:
>
>
>AIR LIQUIDE Deutschland GmbH
>Kunden-Service-Center Spezialgase
>Bataverstraße 47
>47809 Krefeld
>Telefon: (02151) 954-254
>Email : spezialgase.ald at airliquide.com
>
>
>
>Its important to order a 50l cylinder, not a 10l cylinder !
>
>Greetings,
>
>Oliver
>
>
>Dr. Oliver Hofnagel
>Max-Planck-Institute of Molecular Physiology
>Electron Microscopy
>Otto-Hahn-Str. 11
>44227 Dortmund
>Germany
>
>
>
>
>________________________________
>Von: 3dem <3dem-bounces at ncmir.ucsd.edu> im Auftrag von Michael Elbaum
><michael.elbaum at weizmann.ac.il>
>Gesendet: Donnerstag, 20. April 2023 16:29:00
>An: r.i.koning at lumc.nl; cornelia.cazey at cssb-hamburg.de;
>3dem at ncmir.ucsd.edu
>Betreff: Re: [3dem] Calling all groups who use cryogen mix from
>Linde-Gas (Germany, maybe Austria, Switzerland?) for plunging
>
>
>Hi.  would it be possible to use two pure tanks and a mixing regulator?
>I think such regulators are used for welding.
>
>Michael
>
>________________________________
>From: 3dem <3dem-bounces at ncmir.ucsd.edu> on behalf of
>r.i.koning at lumc.nl <r.i.koning at lumc.nl>
>Sent: Thursday, April 20, 2023 5:15:10 PM
>To: cornelia.cazey at cssb-hamburg.de; 3dem at ncmir.ucsd.edu
>Subject: Re: [3dem] Calling all groups who use cryogen mix from
>Linde-Gas (Germany, maybe Austria, Switzerland?) for plunging
>
>
>Dear Cornelia,
>
>
>
>Our most recent order of ethane/propane also did not come through from
>Line because of the same reason. We will have to do with ethane only
>for now but I prefer the mixture for obvious reasons, though we do
>manage with ethane only. We did not look for nor found another vendor.
>
>
>
>Best regards,
>
>
>
>Roman
>
>
>
>Dr. R.I.Koning
>
>
>
>Leiden University Medical Center, Cell and Chemical Biology, Electron
>Microscopy
>
>Postal Zone S1-P, P.O.Box 9600, 2300 RC, Leiden, The Netherlands
>
>
>
>visiting address. Einthovenweg 20, Building 2, zone S1-P, room R-90-21
>
>tel. (31) 71 526 9296 fax. (+31) 71 526 8270 mail.
>r.i.koning at lumc.nl<mailto:r.i.koning at lumc.nl> web. Electron Microscopy
>Facility<https://urldefense.com/v3/__https://ccb.lumc.nl/electron-microscopy-198__;!!Mih3wA!GxgfkasxXThNdN_k1a9iqn0d9uls-PLaHnrrJAIhFXNITn6ehlzCx_U6V-rQFPvgm8pSGK1CJ1Vd55FxuUK3uvc$>
>
>
>
>From: 3dem <3dem-bounces at ncmir.ucsd.edu> On Behalf Of Cazey, Cornelia
>Sent: donderdag 20 april 2023 15:52
>To: 3dem at ncmir.ucsd.edu
>Subject: [3dem] Calling all groups who use cryogen mix from Linde-Gas
>(Germany, maybe Austria, Switzerland?) for plunging
>
>
>
>Dear list participants,
>
>
>
>Our lab is in Germany, and this is a very particular question relating
>to a German supplier, so apologies for addressing the entire list, but
>it seemed the easiest way to find groups who may be able to help.
>
>
>
>We recently learned that Linde-Gas, our supplier for the cryogen mix we
>use in our facility (37% ethane, rest propane) is no longer able to
>supply this mixture due to the overall difficulties in the production,
>age and poor state of their necessary equipment.
>
>
>
>I spoke with the supplier yesterday and while they would like to
>continue to offer the mix, they feel there is not enough market to make
>it feasible. I am now looking for info from groups who are using a
>cryogen mix rather than pure ethane for plunging (I am not sure, but
>relatively convinced that the process would be the same for any kind of
>mixture, so I guess the exact percentages won't matter too much) and
>have previously sourced this from Linde-Gas, and might be or have
>already been affected by this decision. If you have been affected, did
>you find a solution, a different supplier or any other alternative you
>are willing to share? Or if you didn't yet know, how much would this
>news affect your ability to work, and would you be interested to try
>and convince Linde-Gas to keep going? I understand the production line
>has already been shut down, but could be revived with enough interest,
>however, it may also come with a price increase (what doesn't).
>
>
>
>Any help would be greatly appreciated, whether info on alternatives or
>willingness to push for the supply. I am happy to collect replies to
>send to the Linde-Gas representative in bulk or, of course, for those
>who prefer to contact him directly, do so themselves and increase the
>pressure to keep going/restart production.
>
>
>
>Thanks a lot for your help!
>
>
>
>Best wishes,
>
>
>
>Cornelia Cazey
>
>
>
>Cornelia Cazey (She/Her)
>
>Cryo-EM Facility
>
>Centre for Structural Systems Biology (CSSB)
>c/o DESY, Building 15
>Notkestr. 85
>22607 Hamburg
>Germany
>
>phone: +49-(0)40-8998-87706
>email:cornelia.cazey at cssb-hamburg.de
>
>"If you don't know it's impossible, it's easier to do" -Neil Gaiman
>
>
>
>Sometimes I may send emails outside of core working hours. I do not
>expect you to read, or respond outside of your own working hours!
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