<html><head><style type="text/css" style="display:none">
<!--
p
{margin-top:0;
margin-bottom:0}
-->
</style></head><body style="zoom: 0%;"><div dir="auto">Hi Oliver,<br><br></div>
<div dir="auto">Thanks a lot for the info. But why do they only supply in 50l cylinders? Or rather why order a 50l cylinder? Economic reasons? <br><br></div>
<div dir="auto">Thanks!<br><br></div>
<div dir="auto">Cornelia</div>
<div class="gmail_quote" >On 25 Apr 2023, at 13:40, Oliver Hofnagel <<a href="mailto:oliver.hofnagel@mpi-dortmund.mpg.de" target="_blank">oliver.hofnagel@mpi-dortmund.mpg.de</a>> wrote:<blockquote class="gmail_quote" style="margin: 0pt 0pt 0pt 0.8ex; border-left: 1px solid rgb(204, 204, 204); padding-left: 1ex;">
<div id="divtagdefaultwrapper" style="font-size:12pt;color:#000000;font-family:Calibri,Helvetica,sans-serif;" dir="ltr">
<div id="divtagdefaultwrapper" dir="ltr" style="font-size: 12pt; color: rgb(0, 0, 0); font-family: Calibri, Helvetica, sans-serif, "EmojiFont", "Apple Color Emoji", "Segoe UI Emoji", NotoColorEmoji, "Segoe UI Symbol", "Android Emoji", EmojiSymbols;">
<p>Dear Cornelia,</p>
<p><br> </p>
<p>here our supplier for 37% ethane / 63% propane 50l cylinder cryogen mix in Germany:</p>
<p><i><br> </i></p>
<p><i><span style="left: 61.45%; top: 24.19%; font-size: 8pt; font-family: sans-serif; transform: scaleX(1.05234);" role="presentation" dir="ltr">AIR LIQUIDE Deutschland GmbH</span><br role="presentation"> <span style="left: 61.45%; top: 25.19%; font-size: 8pt; font-family: sans-serif; transform: scaleX(1.02532);" role="presentation" dir="ltr">Kunden-Service-Center Spezialgase</span><br role="presentation"> <span style="left: 61.45%; top: 27.18%; font-size: 8pt; font-family: sans-serif; transform: scaleX(1.06881);" role="presentation" dir="ltr">Bataverstraße 47</span><br role="presentation"> <span style="left: 61.45%; top: 28.18%; font-size: 8pt; font-family: sans-serif; transform: scaleX(1.11559);" role="presentation" dir="ltr">47809 Krefeld</span><br role="presentation"> <span style="left: 61.45%; top: 29.18%; font-size: 8pt; font-family: sans-serif; transform: scaleX(1.1413);" role="presentation" dir="ltr">Telefon: (02151) 954-254</span><br role="presentation"> </i><span style="left: 61.45%; top: 30.18%; font-size: 8pt; font-family: sans-serif; transform: scaleX(1.04558);" role="presentation" dir="ltr"><i>Email : spezialgase.ald@airliquide.com</i></span></p>
<p><span style="left: 61.45%; top: 30.18%; font-size: 8pt; font-family: sans-serif; transform: scaleX(1.04558);" role="presentation" dir="ltr"><br> </span></p>
<p><span style="left: 61.45%; top: 30.18%; font-size: 8pt; font-family: sans-serif; transform: scaleX(1.04558);" role="presentation" dir="ltr"><br> </span></p>
<p><span style="left: 61.45%; top: 30.18%; font-size: 12pt; font-family: sans-serif; transform: scaleX(1.04558);" role="presentation" dir="ltr">Its important to order a 50l cylinder, not a 10l cylinder !<br> </span></p>
<p><span style="left: 61.45%; top: 30.18%; font-size: 12pt; font-family: sans-serif; transform: scaleX(1.04558);" role="presentation" dir="ltr">Greetings,</span></p>
<p><span style="left: 61.45%; top: 30.18%; font-size: 12pt; font-family: sans-serif; transform: scaleX(1.04558);" role="presentation" dir="ltr">Oliver <br> </span></p>
<p><span style="left: 61.45%; top: 30.18%; font-size: 12pt; font-family: sans-serif; transform: scaleX(1.04558);" role="presentation" dir="ltr"><br> </span></p>
<p><span style="left: 61.45%; top: 30.18%; font-size: 12pt; font-family: sans-serif; transform: scaleX(1.04558);" role="presentation" dir="ltr"></span></p>
<div>
<span style="font-size: 8pt;">Dr. Oliver Hofnagel</span>
<br>
<span style="font-size: 8pt;">Max-Planck-Institute of Molecular Physiology</span>
<br>
<span style="font-size: 8pt;">Electron Microscopy</span>
<br>
<span style="font-size: 8pt;">Otto-Hahn-Str. 11</span>
<br>
<span style="font-size: 8pt;">44227 Dortmund</span>
<br>
<span style="font-size: 8pt;">Germany</span>
<br>
<span style="font-size: 8pt;"><br> </span>
</div>
<br>
<p></p>
<p><br> </p>
</div>
<hr style="display:inline-block; width:98%" tabindex="-1">
<div id="divRplyFwdMsg" dir="ltr">
<font style="font-size:11pt" face="Calibri, sans-serif" color="#000000"><b>Von:</b> 3dem <3dem-bounces@ncmir.ucsd.edu> im Auftrag von Michael Elbaum <michael.elbaum@weizmann.ac.il><br> <b>Gesendet:</b> Donnerstag, 20. April 2023 16:29:00<br> <b>An:</b> r.i.koning@lumc.nl; cornelia.cazey@cssb-hamburg.de; 3dem@ncmir.ucsd.edu<br> <b>Betreff:</b> Re: [3dem] Calling all groups who use cryogen mix from Linde-Gas (Germany, maybe Austria, Switzerland?) for plunging</font>
<div>
</div>
</div>
<div>
<div id="divtagdefaultwrapper" dir="ltr" style="font-size: 12pt; color: rgb(0, 0, 0); font-family: Calibri, Helvetica, sans-serif, "EmojiFont", "Apple Color Emoji", "Segoe UI Emoji", NotoColorEmoji, "Segoe UI Symbol", "Android Emoji", EmojiSymbols;">
<p>Hi. would it be possible to use two pure tanks and a mixing regulator? I think such regulators are used for welding. <br> </p>
<p>Michael<br> </p>
</div>
<hr style="display:inline-block; width:98%" tabindex="-1">
<div id="divRplyFwdMsg" dir="ltr">
<font style="font-size:11pt" face="Calibri, sans-serif" color="#000000"><b>From:</b> 3dem <3dem-bounces@ncmir.ucsd.edu> on behalf of r.i.koning@lumc.nl <r.i.koning@lumc.nl><br> <b>Sent:</b> Thursday, April 20, 2023 5:15:10 PM<br> <b>To:</b> cornelia.cazey@cssb-hamburg.de; 3dem@ncmir.ucsd.edu<br> <b>Subject:</b> Re: [3dem] Calling all groups who use cryogen mix from Linde-Gas (Germany, maybe Austria, Switzerland?) for plunging</font>
<div>
</div>
</div>
<div>
<div style="">
<p style="margin: 0cm; font-size: 11pt; font-family: "Calibri", sans-serif;"><span style="">Dear Cornelia, </span></p>
<p style="margin: 0cm; font-size: 11pt; font-family: "Calibri", sans-serif;"><span style=""> </span></p>
<p style="margin: 0cm; font-size: 11pt; font-family: "Calibri", sans-serif;"><span style="" lang="EN-US">Our most recent order of ethane/propane also did not come through from Line because of the same reason. We will have to do with ethane only for now but I prefer the mixture for obvious reasons, though we do manage with ethane only. We did not look for nor found another vendor.</span></p>
<p style="margin: 0cm; font-size: 11pt; font-family: "Calibri", sans-serif;"><span style="" lang="EN-US"> </span></p>
<p style="margin: 0cm; font-size: 11pt; font-family: "Calibri", sans-serif;"><span style="" lang="EN-US">Best regards,</span></p>
<p style="margin: 0cm; font-size: 11pt; font-family: "Calibri", sans-serif;"><span style="" lang="EN-US"> </span></p>
<p style="margin: 0cm; font-size: 11pt; font-family: "Calibri", sans-serif;"><span style="" lang="EN-US">Roman</span></p>
<p style="margin: 0cm; font-size: 11pt; font-family: "Calibri", sans-serif;"><span style="" lang="EN-US"> </span></p>
<div>
<p style="text-align: center; margin: 0cm; font-size: 11pt; font-family: "Calibri", sans-serif;" align="center"> <b><span style="font-size:10.0pt; font-family:"Arial",sans-serif; color:gray" lang="EN-US">Dr. R.I.Koning</span></b></p>
<p style="text-align: center; margin: 0cm; font-size: 11pt; font-family: "Calibri", sans-serif;" align="center"> <span style="font-size:8.0pt; color:gray" lang="EN-US"> </span></p>
<p style="text-align: center; margin: 0cm; font-size: 11pt; font-family: "Calibri", sans-serif;" align="center"> <span style="font-size:8.0pt; font-family:"Arial",sans-serif; color:gray" lang="EN-GB">Leiden University Medical Center, Cell and Chemical Biology, Electron Microsc</span><span style="font-size:8.0pt; font-family:"Arial",sans-serif; color:gray" lang="EN-US">opy</span><span style="font-size:8.0pt; color:gray" lang="EN-US"></span></p>
<p style="text-align: center; margin: 0cm; font-size: 11pt; font-family: "Calibri", sans-serif;" align="center"> <span style="font-size:8.0pt; font-family:"Arial",sans-serif; color:gray" lang="EN-US">Postal Zone S1-P, P.O.Box 9600, 2300 RC, Leiden, The Netherlands</span></p>
<p style="text-align: center; margin: 0cm; font-size: 11pt; font-family: "Calibri", sans-serif;" align="center"> <span style="font-size:8.0pt; font-family:"Arial",sans-serif; color:gray" lang="EN-US"> </span></p>
<p style="text-align: center; margin: 0cm; font-size: 11pt; font-family: "Calibri", sans-serif;" align="center"> <b><span style="font-size:8.0pt; font-family:"Arial",sans-serif; color:gray" lang="EN-US">visiting address.</span></b><span style="font-size:8.0pt; font-family:"Arial",sans-serif; color:gray" lang="EN-US"> Einthovenweg 20, Building 2, zone S1-P, room R-90-21</span></p>
<p style="text-align: center; margin: 0cm; font-size: 11pt; font-family: "Calibri", sans-serif;" align="center"> <b><span style="font-size:8.0pt; font-family:"Arial",sans-serif; color:gray" lang="EN-US">tel.</span></b><span style="font-size:8.0pt; font-family:"Arial",sans-serif; color:gray" lang="EN-US"> (31) 71 526 9296 <b>fax.</b> (+31) 71 526 8270 <b>mail.</b></span><b><span style="font-size:8.0pt; font-family:"Arial",sans-serif; color:#4472C4" lang="EN-US"> </span></b><span style="color:#4472C4" lang="EN-US"><a href="mailto:r.i.koning@lumc.nl"><span style="font-size:8.0pt; font-family:"Arial",sans-serif; color:#4472C4">r.i.koning@lumc.nl</span></a></span><span style="font-size:8.0pt; font-family:"Arial",sans-serif; color:gray" lang="EN-US"> <b>web. </b></span><span style="font-size:8.0pt; font-family:"Arial",sans-serif; color:#4472C4" lang="EN-US"><a href="https://urldefense.com/v3/__https://ccb.lumc.nl/electron-microscopy-198__;!!Mih3wA!GxgfkasxXThNdN_k1a9iqn0d9uls-PLaHnrrJAIhFXNITn6ehlzCx_U6V-rQFPvgm8pSGK1CJ1Vd55FxuUK3uvc$"><span style="color:#4472C4">Electron Microscopy Facility</span></a></span><span style="font-size:8.0pt; font-family:"Times New Roman",serif; color:gray" lang="EN-US"></span></p>
</div>
<p style="margin: 0cm; font-size: 11pt; font-family: "Calibri", sans-serif;"><span style=""> </span></p>
<div>
<div style="border:none; border-top:solid #E1E1E1 1.0pt; padding:3.0pt 0cm 0cm 0cm">
<p style="margin: 0cm; font-size: 11pt; font-family: "Calibri", sans-serif;"><b><span lang="EN-US">From:</span></b><span lang="EN-US"> 3dem <3dem-bounces@ncmir.ucsd.edu> <b>On Behalf Of </b>Cazey, Cornelia<br> <b>Sent:</b> donderdag 20 april 2023 15:52<br> <b>To:</b> 3dem@ncmir.ucsd.edu<br> <b>Subject:</b> [3dem] Calling all groups who use cryogen mix from Linde-Gas (Germany, maybe Austria, Switzerland?) for plunging</span></p>
</div>
</div>
<p style="margin: 0cm; font-size: 11pt; font-family: "Calibri", sans-serif;"> </p>
<div>
<div>
<p style="margin: 0cm; font-size: 11pt; font-family: "Calibri", sans-serif;"><span style="font-size:12.0pt; font-family:"Arial",sans-serif; color:black">Dear list participants,</span></p>
</div>
<div>
<p style="margin: 0cm; font-size: 11pt; font-family: "Calibri", sans-serif;"><span style="font-size:12.0pt; font-family:"Arial",sans-serif; color:black"> </span></p>
</div>
<div>
<p style="margin: 0cm; font-size: 11pt; font-family: "Calibri", sans-serif;"><span style="font-size:12.0pt; font-family:"Arial",sans-serif; color:black">Our lab is in Germany, and this is a very particular question relating to a German supplier, so apologies for addressing the entire list, but it seemed the easiest way to find groups who may be able to help.</span></p>
</div>
<div>
<p style="margin: 0cm; font-size: 11pt; font-family: "Calibri", sans-serif;"><span style="font-size:12.0pt; font-family:"Arial",sans-serif; color:black"> </span></p>
</div>
<div>
<p style="margin: 0cm; font-size: 11pt; font-family: "Calibri", sans-serif;"><span style="font-size:12.0pt; font-family:"Arial",sans-serif; color:black">We recently learned that Linde-Gas, our supplier for the cryogen mix we use in our facility (37% ethane, rest propane) is no longer able to supply this mixture due to the overall difficulties in the production, age and poor state of their necessary equipment.</span></p>
</div>
<div>
<p style="margin: 0cm; font-size: 11pt; font-family: "Calibri", sans-serif;"><span style="font-size:12.0pt; font-family:"Arial",sans-serif; color:black"> </span></p>
</div>
<div>
<p style="margin: 0cm; font-size: 11pt; font-family: "Calibri", sans-serif;"><span style="font-size:12.0pt; font-family:"Arial",sans-serif; color:black">I spoke with the supplier yesterday and while they would like to continue to offer the mix, they feel there is not enough market to make it feasible. I am now looking for info from groups who are using a cryogen mix rather than pure ethane for plunging <span style="background:white">(I am not sure, but relatively convinced that the process would be the same for any kind of mixture, so I guess the exact percentages won't matter too much) and</span> have previously sourced this from Linde-Gas, and might be or have already been affected by this decision. If you have been affected, did you find a solution, a different supplier or any other alternative you are willing to share? Or if you didn't yet know, how much would this news affect your ability to work, and would you be interested to try and convince Linde-Gas to keep going? I understand the production line has already been shut down, but could be revived with enough interest, however, it may also come with a price increase (what doesn't). </span></p>
</div>
<div>
<p style="margin: 0cm; font-size: 11pt; font-family: "Calibri", sans-serif;"><span style="font-size:12.0pt; font-family:"Arial",sans-serif; color:black"> </span></p>
</div>
<div>
<p style="margin: 0cm; font-size: 11pt; font-family: "Calibri", sans-serif;"><span style="font-size:12.0pt; font-family:"Arial",sans-serif; color:black">Any help would be greatly appreciated, whether info on alternatives or willingness to push for the supply. I am happy to collect replies to send to the Linde-Gas representative in bulk or, of course, for those who prefer to contact him directly, do so themselves and increase the pressure to keep going/restart production.</span></p>
</div>
<div>
<p style="margin: 0cm; font-size: 11pt; font-family: "Calibri", sans-serif;"><span style="font-size:12.0pt; font-family:"Arial",sans-serif; color:black"> </span></p>
</div>
<div>
<p style="margin: 0cm; font-size: 11pt; font-family: "Calibri", sans-serif;"><span style="font-size:12.0pt; font-family:"Arial",sans-serif; color:black">Thanks a lot for your help!</span></p>
</div>
<div>
<p style="margin: 0cm; font-size: 11pt; font-family: "Calibri", sans-serif;"><span style="font-size:12.0pt; font-family:"Arial",sans-serif; color:black"> </span></p>
</div>
<div>
<p style="margin: 0cm; font-size: 11pt; font-family: "Calibri", sans-serif;"><span style="font-size:12.0pt; font-family:"Arial",sans-serif; color:black">Best wishes,</span></p>
</div>
<div>
<p style="margin: 0cm; font-size: 11pt; font-family: "Calibri", sans-serif;"><span style="font-size:12.0pt; font-family:"Arial",sans-serif; color:black"> </span></p>
</div>
<div>
<p style="margin: 0cm; font-size: 11pt; font-family: "Calibri", sans-serif;"><span style="font-size:12.0pt; font-family:"Arial",sans-serif; color:black">Cornelia Cazey</span></p>
</div>
<div>
<p style="margin: 0cm; font-size: 11pt; font-family: "Calibri", sans-serif;"><span style="font-size:12.0pt; font-family:"Arial",sans-serif; color:black"> </span></p>
</div>
<div>
<p style="margin: 0cm; font-size: 11pt; font-family: "Calibri", sans-serif;"><span style="font-size:10.0pt; font-family:"Arial",sans-serif; color:black">Cornelia Cazey (She/Her)</span><span style="font-size:12.0pt; font-family:"Arial",sans-serif; color:black"></span></p>
</div>
<div>
<div>
<p style="margin: 0cm; font-size: 11pt; font-family: "Calibri", sans-serif;"><span style="font-size:10.0pt; font-family:"Arial",sans-serif; color:black">Cryo-EM Facility</span><span style="font-size:12.0pt; font-family:"Arial",sans-serif; color:black"></span></p>
</div>
<div>
<p style="margin: 0cm; font-size: 11pt; font-family: "Calibri", sans-serif;"><span style="font-size:10.0pt; font-family:"Arial",sans-serif; color:black">Centre for Structural Systems Biology (CSSB)</span><span style="font-size:12.0pt; font-family:"Arial",sans-serif; color:black"><br> </span><span style="font-size:10.0pt; font-family:"Arial",sans-serif; color:black">c/o DESY, Building 15</span><span style="font-size:12.0pt; font-family:"Arial",sans-serif; color:black"><br> </span><span style="font-size:10.0pt; font-family:"Arial",sans-serif; color:black">Notkestr. 85</span><span style="font-size:12.0pt; font-family:"Arial",sans-serif; color:black"><br> </span><span style="font-size:10.0pt; font-family:"Arial",sans-serif; color:black">22607 Hamburg</span><span style="font-size:12.0pt; font-family:"Arial",sans-serif; color:black"><br> </span><span style="font-size:10.0pt; font-family:"Arial",sans-serif; color:black">Germany</span><span style="font-size:12.0pt; font-family:"Arial",sans-serif; color:black"></span></p>
</div>
<div>
<p style="margin: 0cm; font-size: 11pt; font-family: "Calibri", sans-serif;"><span style="font-size:12.0pt; font-family:"Arial",sans-serif; color:black"><br> </span><span style="font-size:10.0pt; font-family:"Arial",sans-serif; color:black">phone: +49-(0)40-8998-87706</span><span style="font-size:12.0pt; font-family:"Arial",sans-serif; color:black"><br> </span><span style="font-size:10.0pt; font-family:"Arial",sans-serif; color:black">email:</span><u><span style="font-size:10.0pt; font-family:"Arial",sans-serif; color:blue">cornelia.cazey@cssb-hamburg.de</span></u><span style="font-size:12.0pt; font-family:"Arial",sans-serif; color:black"><br> <br> </span><em><span style="font-size:10.0pt; font-family:"Arial",sans-serif; color:black">"If you don't know it's impossible, it's easier to do"</span></em><span style="font-size:10.0pt; font-family:"Arial",sans-serif; color:black"> -Neil Gaiman</span><span style="font-size:12.0pt; font-family:"Arial",sans-serif; color:black"></span></p>
</div>
<div>
<p style="margin: 0cm; font-size: 11pt; font-family: "Calibri", sans-serif;"><span style="font-size:12.0pt; font-family:"Arial",sans-serif; color:black"> </span></p>
</div>
<div>
<p style="margin: 0cm; font-size: 11pt; font-family: "Calibri", sans-serif;"><span style="font-size:10.5pt; font-family:"Arial",sans-serif; color:#333333; background:#FDFDFD">Sometimes I may send emails outside of core working hours. I do not expect you to read, or respond outside of your own working hours!</span><span style="font-size:12.0pt; font-family:"Arial",sans-serif; color:black"></span></p>
</div>
</div>
</div>
</div>
</div>
</div>
</div></blockquote></div></body></html>